Accurate prediction of drug absorption, metabolism, and toxicity (ADME/Tox) is essential for successful drug development. The intestinal epithelium plays a central role in regulating systemic drug exposure - but conventional 2D cell lines and static inserts fail to replicate its physiological complexity.
This application note demonstrates how MIMETAS’ OrganoReady® Colon Organoid model overcomes these limitations. Derived from human adult stem cells and cultured in the OrganoPlate® 3-lane microfluidic platform, this 3D system recreates the architecture and function of the human colon epithelium under dynamic flow conditions.
Key Highlights
- Quantify efflux transporter activity (P-gp, BCRP) using fluorescence-based assays and reference inhibitors
- Measure CYP3A4 metabolic enzyme function, including induction and inhibition responses
- Evaluate barrier integrity and recovery under physiological perfusion
- Integrate multimodal readouts - fluorescence, luminescence, and imaging - on the Tecan Spark® Cyto platform
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