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      • Layered tissues with perfused tubules in the absence of artificial membranes form the heart of our permeability and transport science. Study cell interactions, permeability, absorption, transport, and transcytosis without physical barriers.

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  • About Mimetas
  • Expand your drug discovery capacity, shoulder to shoulder with our scientific team. Proprietary human disease biology in the OrganoPlate® platform. Together we make the therapeutics of tomorrow.
  • We create novel human tissue and disease models in the OrganoPlate® platform. Our experts are looking forward to developing assays according to your specifications.
  • Get robust compound data in human tissue models through our services. With proven pheno­typic assays in the OrganoPlate® platform, we support your drug discovery and development needs.
  • We offer several services to support your drug discovery and development needs. Find the overview here.
  • Start today with 3D tissue modeling
    Kickstart your first experiments with the OrganoPlate® through our OrganoStart packages.
  • OrganoFlow®
    Driving precisely-controlled perfusion flow in the OrganoPlate® platform

  • OrganoTEER®
    Enabling fast, automated, and impedance-based TEER measurements in OrganoPlate®. Assay 40 or 64 tissue culture chips with a few clicks, in less than two minutes.

  • Start today with 3D tissue modeling
    Kickstart your experiments with the most sophisticated 3D tissue culture platform. Find out which training fits your 3D tissue modeling needs best.
  • OrganoPlate® is the solution for all in vitro tissue culture applications. Explore the entire OrganoPlate® family and its dedicated instruments here.

  • Thinking of using OrganoPlate for your research? Request a quote for the product(s) of your interest.

  • Layered tissues with perfused tubules in the absence of artificial membranes form the heart of our permeability and transport science. Study cell interactions, permeability, absorption, transport, and transcytosis without physical barriers.

  • Co-culture layered & structured tissues without artificial membranes with perfect imaging, to study barrier-free cellular interactions, cell-cell signaling, and migration.

  • Evaluate the effect of chemotactic triggers or cells on the migration of cells through an extracellular matrix.

  • Membrane-free microvascular formation and growth through an extracellular matrix (ECM).

  • The missing link in tissue culture: add perfusable human vasculature to your tissue models, and recreate sophisticated microenvironments with OrganoPlate® Graft.

  • OrganoPlate® enables you to study relevant 3D tissue biology by incorporating perfused tubules, co-culture, and full control over the tissue microenvironment. Find the overview of applications here.

  • Visit our Knowledge Center to get up to speed with 3D tissue culture and to learn how OrganoPlate® supports your research needs.

    Read our publications, application notes, watch our webinars, or check out the supporting protocols and brochures. All compiled for you, by our scientists.

  • Get inspired by peer-reviewed publications of our scientists, partners, and customers around the globe.

  • Get inspired by research done by our scientists, partners, and customers around the globe.

  • Giving you some food for thought. Read our blogs to learn more about 3D tissue culture, research backgrounds, developments, and its future outlook.
  • Kickstart your experiments with the most sophisticated 3D tissue culture platform. Find out which training fits your 3D tissue modeling needs best.
  • Any support questions about purchasing, products, or 3D tissue culture and analysis? Get in touch with our experts.
  • Thinking of using OrganoPlate for your research? Request a quote for the product(s) of your interest.

  • Frequently asked questions answered by our experts. Find here the answer you're looking for.
  • Learn about our mission, vision, the history of the company, and find out what we mean with MIMETAS-do.
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OrganoReady® Blood-Brain Barrier HBMEC

40 or 64 ready-to-use primary human microvasculature endothelial cell tubules for drug exposure, transport, and permeability studies.

Do you want to perform experiments in human-relevant​ and reproducible Blood-Brain Barrier 3D models, ​but don’t have the time to optimize the conditions? ​With the OrganoReady® BBB HBMEC, you can study the BBB like never before. In contrast to other cell lines or iPSC-based BBB models, this platform is made with primary HBMEC cells that expresses all relevant markers, transporters, receptors and enzymes.

Built on the OrganoPlate® 3-lane 40 or 64, ​you can immediately de-risk your compounds toxicity by​ measuring barrier integrity with fluorescent dye leakage,​ or in a faster, more sensitive method with the OrganoTEER®.

OrganoReady® BBB HBMEC features:​

  • 40, or 64 ready-to use HBMEC perfused tubules
  • OrganoMedium HBMEC-BM ​
  • HBMEC cell line: Human Brain Microvascular Endothelial Cells
  • ECM: Collagen-I​ (Rat Tail)

Assess the permeability and active transport of your ​compounds with direct apical and basal access.​ Add your neuronal component to obtain a complete​ neurovascular unit in an optimized, and consistent ​batch of human primary Blood-Brain Barrier cells ​grown against a validated batch of Collagen-I.

 

Our OrganoReady® Family

Product details

Meet the OrganoReady® BBB HBMEC


TEER evaluation under physiological conditions
Assess barrier integrity quantitatively and robustly in less than 2 minutes for all 40 or 64 replicates ​with the OrganoTEER®.

We create the model so you can focus on your assays
Preparation and QC of OrganoReady BBB HBMEC are done by our experts. Start screening wiht the OrganoReady BBB HBMEC 1 day after arrival for up to 7 days.

Get inspired by a wide range of possible applications
Study (drug-induced) toxicity, inflammation, or quantify drug transport to the CNS.
Use the model for disease modeling or for fundamental research on the BBB.

Apical and basal access
Polarized and perfusable HBMEC tubules, allowing barrier integrity (TEER), transporter (e.g. P-gp), and permeability assays.

Assay versatility
Sampling for off-plate analysis of RNA, DNA, proteins, and metabolites, or perform live-cell as well as fixed staining.

Save your cells and time
With less than 100 µl per perfusion lane and ​no daily media changes.

Dynamic and robust barrier integrity assessment

 

Request Quote Product flyer

Efficiency at its finest. Meet the OrganoReady.

The OrganoReady® BBB HBMEC plate offers a fast and guaranteed way to obtain data in a relevant Blood Brain Barrier model. The live cell tubules, after being brought back to culture in a few easy steps, are ready to use in less than 24 hours.

In this video, our cell culture analyst unboxes the BBB HBMEC model, showing you how easy it is to get the OrganoReady plates into culture. Drop the specialized instruments. It's all ready to go. Are you ready to take your cell culture to the next level?

Our OrganoReady® family

How the OrganoReady is used in routine screens:

“The OrganoReady model has been very useful for fast screening of novel gene therapy related technologies in our company. The membrane-free fluidics is a high throughput screening tool to monitor a transfer of antibodies through the blood brain barrier.” 

Svetlana Pasteuning, VectorY B.V., Netherlands  ​

Want to know more?


Get up to speed with 3D tissue culture and learn how OrganoPlate® supports your research needs.

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