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  • Technology
  • Services
    • Drug Development Partnerships
      • Expand your drug discovery capacity, shoulder to shoulder with our scientific team. Proprietary human disease biology in the OrganoPlate® platform. Together we make the therapeutics of tomorrow.
    • Custom Model & Assay Development
      • We create novel human tissue and disease models in the OrganoPlate® platform. Our experts are looking forward to developing assays according to your specifications.
    • Compound profiling & Screening
      • Get robust compound data in human tissue models through our services. With proven pheno­typic assays in the OrganoPlate® platform, we support your drug discovery and development needs.
    • All Services
      • We offer several services to support your drug discovery and development needs. Find the overview here.
  • Products
  • Applications
    • Perfused Tubules

      • Layered tissues with perfused tubules in the absence of artificial membranes form the heart of our permeability and transport science. Study cell interactions, permeability, absorption, transport, and transcytosis without physical barriers.

    • Cell-Cell Interactions

      • Co-culture layered & structured tissues without artificial membranes with perfect imaging, to study barrier-free cellular interactions, cell-cell signaling, and migration.

    • 3D Cell Migration

      • Evaluate the effect of chemotactic triggers or cells on the migration of cells through an extracellular matrix.

    • Angiogenesis

      • Membrane-free microvascular formation and growth through an extracellular matrix (ECM).

    • Vascularization

      • The missing link in tissue culture: add perfusable human vasculature to your tissue models, and recreate sophisticated microenvironments with OrganoPlate® Graft.

    • All Applications

      • OrganoPlate® enables you to study relevant 3D tissue biology by incorporating perfused tubules, co-culture, and full control over the tissue microenvironment. Find the overview of applications here.

  • Resources & Support
    • Knowledge Center

      • Visit our Knowledge Center to get up to speed with 3D tissue culture and to learn how OrganoPlate® supports your research needs.

        Read our publications, application notes, watch our webinars, or check out the supporting protocols and brochures. All compiled for you, by our scientists.

    • Publications

      • Get inspired by peer-reviewed publications of our scientists, partners, and customers around the globe.

    • Case Studies

      • Get inspired by research done by our scientists, partners, and customers around the globe.

    • Blogs
      • Giving you some food for thought. Read our blogs to learn more about 3D tissue culture, research backgrounds, developments, and its future outlook.
    • Workshops & Trainings
      • Kickstart your experiments with the most sophisticated 3D tissue culture platform. Find out which training fits your 3D tissue modeling needs best.
    • Technical Support
      • Any support questions about purchasing, products, or 3D tissue culture and analysis? Get in touch with our experts.
    • Request a quote

      • Thinking of using OrganoPlate for your research? Request a quote for the product(s) of your interest.

    • FAQ
      • Frequently asked questions answered by our experts. Find here the answer you're looking for.
  • Knowledge Center
  • About Mimetas
  • Expand your drug discovery capacity, shoulder to shoulder with our scientific team. Proprietary human disease biology in the OrganoPlate® platform. Together we make the therapeutics of tomorrow.
  • We create novel human tissue and disease models in the OrganoPlate® platform. Our experts are looking forward to developing assays according to your specifications.
  • Get robust compound data in human tissue models through our services. With proven pheno­typic assays in the OrganoPlate® platform, we support your drug discovery and development needs.
  • We offer several services to support your drug discovery and development needs. Find the overview here.
  • Start today with 3D tissue modeling
    Kickstart your first experiments with the OrganoPlate® through our OrganoStart packages.

  • OrganoFlow®
    Driving precisely-controlled perfusion flow in the OrganoPlate® platform

  • OrganoTEER®
    Enabling fast, automated, and impedance-based TEER measurements in OrganoPlate®. Assay 40 or 64 tissue culture chips with a few clicks, in less than two minutes.

  • Start today with 3D tissue modeling
    Kickstart your experiments with the most sophisticated 3D tissue culture platform. Find out which training fits your 3D tissue modeling needs best.

  • OrganoPlate® is the solution for all in vitro tissue culture applications. Explore the entire OrganoPlate® family and its dedicated instruments here.

  • Thinking of using OrganoPlate for your research? Request a quote for the product(s) of your interest.

  • Layered tissues with perfused tubules in the absence of artificial membranes form the heart of our permeability and transport science. Study cell interactions, permeability, absorption, transport, and transcytosis without physical barriers.

  • Co-culture layered & structured tissues without artificial membranes with perfect imaging, to study barrier-free cellular interactions, cell-cell signaling, and migration.

  • Evaluate the effect of chemotactic triggers or cells on the migration of cells through an extracellular matrix.

  • Membrane-free microvascular formation and growth through an extracellular matrix (ECM).

  • The missing link in tissue culture: add perfusable human vasculature to your tissue models, and recreate sophisticated microenvironments with OrganoPlate® Graft.

  • OrganoPlate® enables you to study relevant 3D tissue biology by incorporating perfused tubules, co-culture, and full control over the tissue microenvironment. Find the overview of applications here.

  • Visit our Knowledge Center to get up to speed with 3D tissue culture and to learn how OrganoPlate® supports your research needs.

    Read our publications, application notes, watch our webinars, or check out the supporting protocols and brochures. All compiled for you, by our scientists.

  • Get inspired by peer-reviewed publications of our scientists, partners, and customers around the globe.

  • Get inspired by research done by our scientists, partners, and customers around the globe.

  • Giving you some food for thought. Read our blogs to learn more about 3D tissue culture, research backgrounds, developments, and its future outlook.
  • Kickstart your experiments with the most sophisticated 3D tissue culture platform. Find out which training fits your 3D tissue modeling needs best.
  • Any support questions about purchasing, products, or 3D tissue culture and analysis? Get in touch with our experts.

  • Thinking of using OrganoPlate for your research? Request a quote for the product(s) of your interest.

  • Frequently asked questions answered by our experts. Find here the answer you're looking for.
  • Learn about our mission, vision, the history of the company, and find out what we mean with MIMETAS-do.
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Knowledge Center


Get up to speed with 3D tissue culture and learn how OrganoPlate® supports your research needs. Read publications from customers and our scientists, watch our webinars, or check our application notes, protocols, and brochures.

Filter on your topic of interest to get an overview of all related resources.

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General: get started with 3D tissue culture
General Protocols
Assay Protocols
All Protocols
OrganoPlate® 2-lane 96
OrganoPlate® 3-lane 40
OrganoPlate® 3-lane 64
OrganoPlate® Graft
OrganoReady Colon Caco-2
OrganoTEER®
OrganoFlow®
Perfused Tubules
Cell-Cell Interactions
3D Cell Migration
Angiogenesis
Vascularization
Imaging
Filter

Publications

2023 Dose and route dependent effects of the mycotoxin deoxynivalenol in a 3D gut-on-a-chip model with flow. Pöschl et al.

2023 A microfluidic-based PDAC organoid system reveals the impact of hypoxia in response to treatment. Geyer et al.

2023 Vascular inflammation on a chip: A scalable platform for trans-endothelial electrical resistance and immune cell migration. Ehlers et al.

2023 Quantify permeability using on-a-chip models in high-throughput applications. Soragni et al.

2022 A 3D Perfusable Platform for In Vitro Culture of Patient Derived Xenografts. Sablatura et al.

2022 Quantifying the transport of biologics across intestinal barrier models in real-time by fluorescent imaging. Weller et al.

2022 High‑throughput 3D microvessel‑on‑a‑chip model to study defective angiogenesis in systemic sclerosis. Kramer et al.

2022 A versatile multiplexed assay to quantify intracellular ROS and cell viability in 3D on-a-chip models. Soragni et al.

2022 Astrocytes regulate vascular endothelial responses to simulated deep space radiation in a human organ-on-a-chip model. Verma et al.

2022 A Three-Dimensional Brain-on-a-Chip Using Human iPSC-Derived GABAergic Neurons and Astrocytes. Liu et al.

2022 In vitro grafting of hepatic spheroids and organoids on a microfluidic vascular bed. Bonanini et al.

2022 An in silico Model of T Cell Infiltration Dynamics Based on an Advanced in vitroSystem to Enhance Preclinical Decision Making in Cancer Immunotherapy. Lewin et al.

2022 Construction and Functional Evaluation of a Three-Dimensional Blood-Brain Barrier Model Equipped With Human Induced Pluripotent Stem Cell-Derived Brain Microvascular Endothelial Cells. Kurosawa et al.
2022 Endothelial inflammation and neutrophil transmigration are modulated by extracellular matrix composition in an inflammation-on-a-chip model. Riddle et al.
2022 Evaluation of immunotherapies improving macrophage anti-tumor response using a microfluidic model. Chernyavska et al.
2022 Development of human-derived, three-dimensional respiratory epithelial tissue constructs with perfusable microvasculature on a high-throughput microfluidics screening platform. Jung et al.
2022 Modelling and Prevention of Acute Kidney Injury through Ischemia and Reperfusion in a Combined Human Renal Proximal Tubule/Blood Vessel-on-a-Chip., Vormann M. et al.

2021 The Release of Inflammatory Mediators from Acid-Stimulated Mesenchymal Stromal Cells Favours Tumour Invasiveness and Metastasis in Osteosarcoma. Evnet et al.

2021 Adoption of organ-on-chip platforms by the pharmaceutical industry, Vulto et al.

2021 Microfluidic Platforms for High-Throughput Pancreatic Ductal Adenocarcinoma Organoid Culture and Drug Screening., Geyer M. et al.

2021 Modeling ischemic stroke in a triculture neurovascular unit on-a-chip, Wevers N. et al.

2021 A Novel In Vitro Membrane Permeability Methodology Using Three-dimensional Caco-2 Tubules in a Microphysiological System which Better Mimics in Vivo Physiological Conditions., Hagiwara Y. et al.

2021 Intestinal Epithelium Tubules on a Chip., Kosim K. et al.

2021 A Microfluidics-Based Screening Tool to Assess the Impact of Blood Plasma Factors on Microvascular Integrity., Junaid A. et al.

2021 Generation of a Glomerular Filtration Barrier on a Glomerulus-on-a-Chip Platform., Perin L. et al.

2021 Long-Lived Human Lymphatic Endothelial Cells to Study Lymphatic Biology and Lymphatic Vessel/Tumor Coculture in a 3D Microfluidic Model., Frenkel N. et al.

2021 High-throughput 3D cell migration assays in organ-on-a-chip technologies., Goubert D. et al.

2021 A Microfluidic 3D Endothelium-on-a-Chip Model to Study Transendothelial Migration of T Cells in Health and Disease., De Haan L. et al.

2021 A 3D Renal Proximal Tubule on Chip Model Phenocopies Lowe Syndrome and Dent II Disease Tubulopathy., Naik S et al.

2021 Human iPSC-derived brain endothelial microvessels in a multi-well format enable permeability screens of anti-inflammatory drugs., Fengler S. et al.

2021 High throughput transepithelial electrical resistance (TEER) measurements on perfused membrane-free epithelia., Nicolas A. et al.

2021 Culture and analysis of kidney tubuloids and perfused tubuloid cells-on-a-chip., Gijzen L. et al.

2021 Implementation of a human renal proximal tubule on a chip for nephrotoxicity and drug interaction studies., Vormann M. et al.

2021 A directional 3D neurite outgrowth model for studying motor axon biology and disease., Spijkers X. et al.

2021 A 3D Microfluidic Liver Model for High Throughput Compound Toxicity Screening in the OrganoPlate®., Bircsak K. et al.
2020 Development of a human primary gut-on-a-chip to model inflammatory processes., Beaurivage C. et al.
2020 Human Retinal Microvasculature-on-a-Chip for Drug Discovery., Ragelle H. et al.
2020 Inducers of the endothelial cell barrier identified through chemogenomic screening in genome-edited hPSC-endothelial cells., Roudnicky F. et al.

2020 Neutrophilic infiltration in organ-on-a-chip model of tissue inflammation., Gjorevski N. et al.

2020 Metabolic response of blood vessels to TNFα., Junaid A. et al.
2020 Direct On-Chip Differentiation of Intestinal Tubules from Induced Pluripotent Stem Cells., Naumovska E. et al.

2020 Robust and Scalable Angiogenesis Assay of Perfused 3D Human iPSC-Derived Endothelium for Anti-Angiogenic Drug Screening., van Duinen V. et al.

2020 An Intestine-on-a-Chip Model of Plug-and-Play Modularity to Study Inflammatory Processes., Gijzen L. et al.

2020 Three-dimensional Brain-On-Chip Model Using Human iPSC-derived GABAergic Neurons and Astrocytes: Butyrylcholinesterase Post-Treatment for Acute Malathion Exposure., PLoS One, Lumei L. et al.
2020 Snake Venom Gland Organoids., Cell, Post Y. et al.
2020 Flow stimulates drug transport in a human kidney proximal tubule-on-a-chip independent of primary cilia. BBA, J. Vriend et al.

2019 Three-dimensional (3D) brain microphysiological system for organophosphates and neurochemical agent toxicity screening. Liu et al.

2019 Development of a Gut-On-A-Chip Model for High Throughput Disease Modeling and Drug Discovery., IJMS, C. Beaurivage et al.
2019 A glomerulus-on-a-chip to recapitulate the human glomerular filtration barrier. Nature Communications. A. Petrosyan et al.
2019 Standardized and Scalable Assay to Study Perfused 3D Angiogenic Sprouting of iPSC-derived Endothelial Cells In Vitro., van Duinen V. et al.
2019 Automated microfluidic cell culture of stem cell derived dopaminergic neurons. Scientific Reports. Khalid I. W. Kane et al.
2019 Tubuloids derived from human adult kidney and urine for personalized disease modeling. Nature Biotech. Frans Schutgens et al.
2019 Differentiation of the human liver progenitor cell line (HepaRG) on a microfluidic?based biochip. Advanced Science. Mi Jang et al.
2018 Determination of the rheological properties of Matrigel for optimum seeding conditions in microfluidic cell cultures, AIP Advances. Kane et al.
2018 3D Cultures of Parkinson's Disease Specific Dopaminergic Neurons for High Content Phenotyping and Drug Testing. Advanced Science, Bolognin et al.
2018 Perfused 3D angiogenic sprouting in a high-throughput in vitro platform. Angiogenesis. Van Duinen et al.
2018 A perfused human blood-brain barrier on-a-chip for high-throughput assessment of barrier function and antibody transport. Fluids Barriers CNS. Wevers et al.
2018 Nephrotoxicity and Kidney Transport Assessment on 3D Perfused Proximal Tubules. AAPS J. Vormann et al.
2018 Screening of Drug-Transporter Interactions in a 3D Microfluidic Renal Proximal Tubule on a Chip. AAPS J. Vriend et al.
2018 Combining Extracellular miRNA Determination with Microfluidic 3D Cell Cultures for the Assessment of Nephrotoxicity: a Proof of Concept Study. AAPS J. Suter-Dick et al.
2018 Mechanistic Investigations of Diarrhea Toxicity Induced by anti-HER2/3 Combination Therapy, Mol. Cancer Ther., Moisan et al.
2018 Study of melatonin-mediated effects on various hepatic inflammatory responses stimulated by IL-6 in a new HepG2-on-a-chip platform. Biomedical Microdevices. Jang et al.
2018 Acoustic Characterization of a Vessel-on-a-Chip Microfluidic System for Ultrasound-Mediated Drug Delivery. IEEE Trans Ultrason Ferroelectr Freq Control. Beekers et al.
2018 Three-dimensional (3D) tetra-culture brain on chip platform for organophosphate toxicity screening. Sci Reports, Koo et al.
2017 96 perfusable blood vessels to study vascular permeability in vitro. Sci. Reports, van Duinen et al.
2017 Therapy response testing of breast cancer in a 3D high-throughput perfused microfluidic platform. BMC Cancer, Lanz et al.
2017 Membrane-free culture and real-time barrier integrity assessment of perfused intestinal epithelium tubes. Nature Commun., Trietsch et al.
2017 An End-User Perspective on Organ-on-a-Chip: Assays and Usability Aspects. Curr. Op. Biom. Eng., Junaid et al.
2016 High-throughput compound evaluation on 3D networks of neurons and glia in a microfluidic platform. Sci. Reports, Wevers et al.
2016 Biology-inspired microphysiological system approaches to solve the prediction dilemma of substance testing. ALTEX, Marx et al.
2016 Kidney-on-a-Chip Technology for Drug-Induced Nephrotoxicity Screening. Trends in Biotech, Wilmer et al.
2015 Differentiation of neuroepithelial stem cells into functional dopaminergic neurons in 3D microfluidic cell culture. Lab on a Chip, Edinson et al.
2015 Microfluidic 3D cell culture: from tools to tissue models. Curr Opin Biotechnol, van Duinen et al.
2015 Morphogens and blood-brain barrier function in health and disease. Tissue Barriers, Wevers at al.
2015 On-chip three-dimensional cell culture in phaseguides improves hepatocyte functions in vitro. Biomicrofluidics 9, Jang et al.
2014 Phaseguides as tunable passive microvalves for liquid routing in complex microfluidic networks. Lab on a Chip, Yildrim et al.

2013 Microfluidic titer plate for stratified 3D cell culture. Lab on a Chip, Trietsch et al.

2011 Phaseguides: a paradigm shift in microfluidic priming and emptying. Lab on a Chip, Vulto et al.

Protocols

General - ECM gel seeding starter’s guide – OrganoPlate® 2-lane 96
General - Medium Change in OrganoPlate®
General - Channel coating in the OrganoPlate®
General - Wet channel seeding technique for channel coating or sequential cell seeding- OrganoPlate®
OrganoPlate® 2-lane 96 - In-gel seeding
OrganoPlate® 2-lane 96 - Tubule seeding
OrganoPlate® 2-lane 96 - Neurons in-gel seeding
OrganoPlate® 3-lane 40 - In-gel seeding
OrganoPlate® 3-lane 40 - Tubule seeding
OrganoPlate® 3-lane 40 - Caco-2 seeding
OrganoPlate® 3-lane 40 - Caco-2/HT29 seeding
OrganoPlate® 3-lane 40 - Angiogenesis
OrganoPlate® 3-lane 64 - Tubule seeding
OrganoPlate® 3-lane 64 - Caco-2 seeding
OrganoPlate® 3-lane 64 - Angiogenesis
OrganoPlate® Graft - Vascular network formation
OrganoPlate® Graft - Tissue placement
OrganoPlate® Graft - Automated tissue placement
OrganoTEER® - Electrode board cleaning
Assay - Neurite outgrowth in the OrganoPlate®
Assay - WST-8 viability assay in the OrganoPlate®
Assay - RNA isolation from OrganoPlate® cultures
Assay - Monocyte adhesion assay in the OrganoPlate®
Assay - Immunostaining - OrganoPlate® 2-lane & 3-lane
Assay - Immunostaining - OrganoPlate® Graft
Assay - Barrier integrity assay - OrganoPlate® 2-lane & 3-lane
Assay - Perfusion assay - OrganoPlate® Graft

Webinars & Roundtables

NEW (2022) An Expert Discussion: Therapeutic Developments and Innovative Strategies in Oncology

(2022) An Expert Discussion: Innovative approaches for therapy development against Inflammatory Bowel Disease

(2022) A novel BBB-on-a-chip Model for Disease Modelling and High-throughput Barrier and Transport Studies

(2022) Enhancing 3D Disease Models: Automated, High-throughput Phenotypic Screening with Organ-on-a-Chip

(2022) Pancreatic Ductal Adenocarcinoma (PDAC) Modeling and Drug Screening with Organ-on-a-Chip

(2021) High throughput gut-on-a-chip models for drug discovery and target validation in inflammatory bowel disease

(2021) An intestine-on-a-chip model of plug-and-play modularity to study inflammatory processes

(2021) 3D Cell migration using a Microfluidic Endothelium-on-a-Chip Model

(2021) Getting started with 3D human tissue models & imaging

(2021) Advances in Modelling the Tumor Microenvironment for Cancer Drug Development
(2021) Toxicity testing in a high-throughput organ-on-a-chip platform
(2021) High throughput phenotypic screening in Organ-on-a-Chip: future or reality?
(2021) Neutrophilic Infiltration in Organ-on-a-Chip Model of Tissue Inflammation
(2021) The OrganoPlate®: A COVID-19 Research Tool

(2020) Getting started with 3D cell models and imaging – all you need to know (EN) (DE) (FR)

(2020) Combining HUB Organoids™ with Organ-on-a-Chip Technology: the Best of Two Worlds

(2020) In vitro Intestine Permeability Models in 4 Days

(2020) Modelling Angiogenesis From Start to Finish in the OrganoPlate®
(2020) Tissue Barriers: Expanding Your Possibilities

(2020) OrganoTEER®: Know Your Barriers. Fast.

(2019) Automated Microfluidic Cell Culture of Stem Cell-derived Dopaminergic Neurons
(2019) Combining Extracellular miRNA Determination with Microfluidic 3D Cell Cultures to Assess Nephrotoxicity

(2019) Screening of renal drug-transporter interactions in a 3D Human Proximal-Tubule-on-a-Chip

(2019) Nephrotoxicity and Kidney Transport Assessment on 3D Perfused Proximal Tubules

(2019) Development of a Glomerulus-on-a-Chip System to Study the Glomerular Filtration Barrier In Vitro

(2019) Perfused 3D Angiogenic Sprouting In A High-Throughput In Vitro Platform

(2019) OrganoPlate® Graft: The Missing Link In Tissue Culture

(2018) Easy and Reliable In Vitro BBB-on-a-Chip

Application Notes

NEW A 3D human blood-brain barrier model in a high throughput platform for disease modeling and drug development

Building a high-throughput and automated liver-on-a-chip model for hepatotoxicity detection

Automation of an organ-on-a-chip assay: automated culture, imaging, and analysis of angiogenesis

Transendothelial Migration of T Cells in A Microfluidic 3D Endothelium-on-a-Chip Model

3D Immune Cell Migration
In collaboration with Molecular Devices

Human renal proximal tubule-on-a-chip for nephrotoxicity and drug interaction studies

Comparison of the OrganoPlate® and the Transwell® platform for in vitro intestinal permeability assays

The OrganoPlate® Graft: in vitro vascularization of human tissues

Investigating the hallmarks of inflammatory bowel disease (IBD) in a 3D perfused multicellular intestine-on-a-chip model

Making Barrier Integrity Assays Easier: Automated Imaging of Intestinal Barrier Function on a High Throughput Organ-on-a-Chip Platform
In collaboration with BioTek.

3D CNS model of iPSCs derived neurons and glia for high-throughput neurotoxicity screening in the OrganoPlate®

High-Content Assay for Morphological Characterization of 3D Neuronal Networks in a Microfluidic Platform
In collaboration with Molecular Devices.

Establishment and characterization of human kidney organoid-derived perfused tubules in Mimetas’ OrganoPlates®

A 3D Human Kidney Nephrotoxicity Model
In collaboration with Sigma Aldrich.

3D image analysis and characterization of angiogenesis in organ-on-a-chip model
In collaboration with Molecular Devices.

Perfused 3D angiogenic sprouting in a high-throughput in vitro platform

Brochures & Flyers

General

NEW Whitepaper: High-throughput phenotypic screening with Organ-on-a-chip: a perfused 3D microfluidic angiogenesis assay

eBook: Building 3D in vitro intestinal permeability models for drug development

Company General: About MIMETAS and offering

Overview OrganoStart,, -Ready, -Services

Kickstarting your advanced 3D tissue cultures (EN) (JP) (CN)

Flyer_OrganoReady BBB HBMEC

OrganoPlates

The OrganoPlate® family: a comparison between plate properties

OrganoPlate® 2-lane 96 specifications

OrganoPlate® 3-lane 40 specifications

OrganoPlate® 3-lane 64 specifications

OrganoPlate® Graft specifications

Instruments
OrganoTEER® specifications
OrganoFlow® manual
Infographics
Infographic_Why OrganoReady Caco-2?
Infographic_Making in vitro 3D Angiogenesis a Reality
Infographic_Better Intestinal Barriers Without Plate Inserts
Infographic_Methods to Assess Barrier Integrity and Permeability in 3D tissue models
Flyer_Automated versus manual placement of spheroids in OrganoPlate® Graft

Posters

Poster - Blood-brain barrier on-a-chip for high-throughput barrier and transport studies
Poster - The OrganoTEER®: A sensitive TEER measurement platform for high-throughput screening of Organs-on-Chips
Poster - A robotized 1546 compound screen in a perfused 3D microfluidic angiogenesis assay

Poster - A 3D T cell migration model to study transendothelial migration in healthy and diseased conditions

Poster - Development of a 3D microphysiological placenta barrier in vitro model

Poster - Modelling T Cell-Vasculature co-culture in a high-throughput microfluidic platform

Poster - Automation and validation of the OrganoPlate® LiverTox for hepatotoxicity detection
Poster - A Tubular Organoid-derived Gut-on-a-chip model suitable for drug development research
 

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