Chat with us, powered by LiveChat
  • Technology
  • Products
    • OrganoPlate® 2-lane 96
      • Supporting up to 96 tissue models on a single plate. Its unique PhaseGuide™ technology enables cells to interact and migrate freely between the two channels of each chip.

    • OrganoPlate® 3-lane 40
      • Supporting up to 40 tissue models on a single plate. Its unique PhaseGuide™ technology enables cells to interact and migrate freely between the three channels of each chip.
    • OrganoPlate® 3-lane 64
      • Supporting up to 64 tissue models on a single plate. Specially designed for automated workflows. Its unique PhaseGuide™ technology enables cells to interact and migrate freely between the three channels of each chip.
    • OrganoPlate® Graft
      • The first in vitro tissue culture platform that allows co-culture of spheroids, organoids, and tumors with a perfused microvascular bed and vascularization of 3D tissues.

    • OrganoReady® Colon Caco-2
      • Offering 38 ready-to-use Caco-2 tubules for drug exposure, transport, and permeability studies.
    • OrganoReady® Blood Vessel HUVEC
      • Offering 38 ready-to-use HUVEC tubules for drug exposure, transport, and permeability studies.
    • OrganoReady® Angiogenesis HUVEC
      • Offering 64 ready-to-sprout HUVEC tubules.
    • OrganoFlow®
      • Driving precisely-controlled perfusion flow in the OrganoPlate® platform
    • OrganoTEER®
      • Enabling fast, automated, and impedance-based TEER measurements in OrganoPlate®. Assay 40 tissue culture chips with a few clicks, in less than one minute.
    • Workshops & Trainings
      • Kickstart your experiments with the most sophisticated 3D tissue culture platform. Find out which training fits your 3D tissue modeling needs best.
    • All Products
      • OrganoPlate® is the solution for all in vitro tissue culture applications. Explore the entire OrganoPlate® family and its dedicated instruments here.

    • Getting Started
      • Start with the most sophisticated 3D tissue culture platform today.

    • Request a quote
      • Thinking of using OrganoPlate for your research? Request a quote for the product(s) of your interest.

  • Services
    • Compound profiling & Screening
      • Get robust compound data in human tissue models through our OrganoServices. With proven pheno­typic assays in the OrganoPlate® platform, we support your drug discovery and development needs.
    • Custom Model & Assay Development
      • We create novel human tissue and disease models in the OrganoPlate® platform. Our experts are looking forward to developing assays according to your specifications.
    • Drug Development Partnerships
      • Expand your drug discovery capacity, shoulder to shoulder with our scientific team. Proprietary human disease biology in the OrganoPlate® platform. Together we make the therapeutics of tomorrow.
    • All Services
      • We offer several services to support your drug discovery and development needs. Find the overview here.
  • Applications
    • Perfused Tubules
      • Layered tissues with perfused tubules in the absence of artificial membranes form the heart of our permeability and transport science. Study cell interactions, permeability, absorption, transport, and transcytosis without physical barriers.

    • Cell-Cell Interactions
      • Co-culture layered & structured tissues without artificial membranes with perfect imaging, to study barrier-free cellular interactions, cell-cell signaling, and migration.

    • 3D Cell Migration
      • Evaluate the effect of chemotactic triggers or cells on the migration of cells through an extracellular matrix.

    • Angiogenesis
      • Membrane-free microvascular formation and growth through an extracellular matrix (ECM).

    • Vascularization
      • The missing link in tissue culture: add perfusable human vasculature to your tissue models, and recreate sophisticated microenvironments with OrganoPlate® Graft.

    • All Applications
      • OrganoPlate® enables you to study relevant 3D tissue biology by incorporating perfused tubules, co-culture, and full control over the tissue microenvironment. Find the overview of applications here.

  • Resources & Support
    • Knowledge Center
      • Visit our Knowledge Center to get up to speed with 3D tissue culture and to learn how OrganoPlate® supports your research needs.

        Read our publications, application notes, watch our webinars, or check out the supporting protocols and brochures. All compiled for you, by our scientists.

    • Publications
      • Get inspired by peer-reviewed publications of our scientists, partners, and customers around the globe.

    • Case Studies
      • Get inspired by research done by our scientists, partners, and customers around the globe.

    • Blogs
      • Giving you some food for thought. Read our blogs to learn more about 3D tissue culture, research backgrounds, developments, and its future outlook.
    • Workshops & Trainings
      • Kickstart your experiments with the most sophisticated 3D tissue culture platform. Find out which training fits your 3D tissue modeling needs best.
    • Technical Support
      • Any support questions about purchasing, products, or 3D tissue culture and analysis? Get in touch with our experts.
    • Request a quote
      • Thinking of using OrganoPlate for your research? Request a quote for the product(s) of your interest.

    • FAQ
      • Frequently asked questions answered by our experts. Find here the answer you're looking for.
  • About Mimetas
  • Supporting up to 96 tissue models on a single plate. Its unique PhaseGuide™ technology enables cells to interact and migrate freely between the two channels of each chip.

  • Supporting up to 40 tissue models on a single plate. Its unique PhaseGuide™ technology enables cells to interact and migrate freely between the three channels of each chip.
  • Supporting up to 64 tissue models on a single plate. Specially designed for automated workflows. Its unique PhaseGuide™ technology enables cells to interact and migrate freely between the three channels of each chip.
  • The first in vitro tissue culture platform that allows co-culture of spheroids, organoids, and tumors with a perfused microvascular bed and vascularization of 3D tissues.

  • Offering 38 ready-to-use Caco-2 tubules for drug exposure, transport, and permeability studies.
  • Offering 38 ready-to-use HUVEC tubules for drug exposure, transport, and permeability studies.
  • Offering 64 ready-to-sprout HUVEC tubules.
  • Driving precisely-controlled perfusion flow in the OrganoPlate® platform
  • Enabling fast, automated, and impedance-based TEER measurements in OrganoPlate®. Assay 40 tissue culture chips with a few clicks, in less than one minute.
  • Kickstart your experiments with the most sophisticated 3D tissue culture platform. Find out which training fits your 3D tissue modeling needs best.
  • OrganoPlate® is the solution for all in vitro tissue culture applications. Explore the entire OrganoPlate® family and its dedicated instruments here.

  • Start with the most sophisticated 3D tissue culture platform today.

  • Thinking of using OrganoPlate for your research? Request a quote for the product(s) of your interest.

  • Get robust compound data in human tissue models through our OrganoServices. With proven pheno­typic assays in the OrganoPlate® platform, we support your drug discovery and development needs.
  • We create novel human tissue and disease models in the OrganoPlate® platform. Our experts are looking forward to developing assays according to your specifications.
  • Expand your drug discovery capacity, shoulder to shoulder with our scientific team. Proprietary human disease biology in the OrganoPlate® platform. Together we make the therapeutics of tomorrow.
  • We offer several services to support your drug discovery and development needs. Find the overview here.
  • Layered tissues with perfused tubules in the absence of artificial membranes form the heart of our permeability and transport science. Study cell interactions, permeability, absorption, transport, and transcytosis without physical barriers.

  • Co-culture layered & structured tissues without artificial membranes with perfect imaging, to study barrier-free cellular interactions, cell-cell signaling, and migration.

  • Evaluate the effect of chemotactic triggers or cells on the migration of cells through an extracellular matrix.

  • Membrane-free microvascular formation and growth through an extracellular matrix (ECM).

  • The missing link in tissue culture: add perfusable human vasculature to your tissue models, and recreate sophisticated microenvironments with OrganoPlate® Graft.

  • OrganoPlate® enables you to study relevant 3D tissue biology by incorporating perfused tubules, co-culture, and full control over the tissue microenvironment. Find the overview of applications here.

  • Visit our Knowledge Center to get up to speed with 3D tissue culture and to learn how OrganoPlate® supports your research needs.

    Read our publications, application notes, watch our webinars, or check out the supporting protocols and brochures. All compiled for you, by our scientists.

  • Get inspired by peer-reviewed publications of our scientists, partners, and customers around the globe.

  • Get inspired by research done by our scientists, partners, and customers around the globe.

  • Giving you some food for thought. Read our blogs to learn more about 3D tissue culture, research backgrounds, developments, and its future outlook.
  • Kickstart your experiments with the most sophisticated 3D tissue culture platform. Find out which training fits your 3D tissue modeling needs best.
  • Any support questions about purchasing, products, or 3D tissue culture and analysis? Get in touch with our experts.
  • Thinking of using OrganoPlate for your research? Request a quote for the product(s) of your interest.

  • Frequently asked questions answered by our experts. Find here the answer you're looking for.
  • Learn about our mission, vision, the history of the company, and find out what we mean with MIMETAS-do.
EN

FAQ


Do you have any questions?
You might find here the answer you're looking for.

Filter on your topic of interest to get the related frequently asked questions.

All
All
Orders & Delivery
OrganoPlate Specifications
Gel loading
Cell culture
Perfusion
Assays
Imaging
Filter

OrganoPlate Specifications

Should I use an OrganoPlate® 2-lane or a 3-lane?

The main difference between the OrganoPlate® 2-lane or 3-lane is the extra channel in the 3-lane. This allows for more culture options such as: creating a gradient, having two types of ECM besides each other, having basal access to a tube, etc. This makes the OrganoPlate® 3-lane a necessary option for doing e.g. transport assays. The OrganoPlate® 2-lane however, is more fit for high-throughput screenings as it has 96 tissue culture chips per plate instead of the 40 tissue culture chips that the OrganoPlate® 3-lane provides.

Check out the OrganoPlate Family and find out which type fits your needs.

Do I need any special equipment for using OrganoPlate®?

The OrganoPlate® is an SBS-standard 384-well plate size, 127.76 x 85.48 x 14.8 mm (l x w x h) (17.3 mm height with lid) and is compatible with the standard laboratory systems (microscope, plate reader).

What material is the OrganoPlate made of?

  • Top plate: virgin polystyrene.
  • Bottom: optical quality 150 μm glass (#1 coverslip thickness)
  • Microfluidics: glass, proprietary polymers, biocompatible and low-compound-absorbing

What size is the OrganoPlate?

The OrganoPlate® is a SBS-standard 384-well plate size: 127.76 x 85.48 x 14.8 mm (l x w x h) (17.3 mm height with lid)

What are the sizes of the channels per type of OrganoPlate?

OrganoPlate® 2-lane:

  • Channel width:
    • Perfusion channel: 400 µm.
    • ECM-gel channel: 400 µm
  • Channels height: 220 µm

OrganoPlate® 3-lane [4004-400-B]:

  • Channel width:
    • Perfusion channels: 300 µm.
    • ECM-gel channel: 350 µm
  • Channels height: 220 µm

You can find this and other product specifications in our Knowledge Center in the Brochures section.

Can I flip my OrganoPlate upside down?

Yes, because the medium in the OrganoPlate is in small wells, capillary forces ensure that the liquid does not leak out when you flip the OrganoPlate, even if there is medium in the OrganoPlate.

Orders & Delivery

How do I order products through the online quote form?

You can fill out the quote form on our website and the next working day our Sales Team will reach out to you with an official quote that meets your request. Once you accept our quote, our Sales Team will guide you further on how you can proceed with ordering.

How will my order be delivered?

We make efforts to deliver our products within 4 (four) weeks after the agreement takes effect. Shipping and delivery is handled by FedEx. Find our Terms & Conditions here.

Can my order be delivered to a different address then my billing address?

Yes, you can always arrange for your order to be delivered to a different address within the same country. You can specify the billing address and delivery address with our Sales Team.

Can my order be delivered to a PO Box address?

Unfortunately, this is not possible.

To which countries does MIMETAS ship orders?

We deliver worldwide.

How can I cancel an order?

Once an order has been placed, it is final and, unfortunately, cannot be canceled. If you no longer want the items, you can send them back as soon as you receive them (within 14 days). You will then be refunded once we have received the items in the original state of the package, unopened and undamaged.

What are the shipping costs?

Area OrganoPlate [QTY 1 - 20] OrganoFlow
The Netherlands 15 EUR 15 EUR
Europe 50 EUR 90 EUR
North America 85 USD 85 USD
South America 85 USD 85 USD
Asia 70 EUR 110 EUR
Africa 110 EUR 110 EUR
Australia 110 EUR 110 EUR


I haven’t received my order, what should I do?

If you have not received the items you ordered within 4 (four) weeks, please contact our Customer Service department through support[at]mimetas.com

I received an item I did not order, what should I do?

If you have received an item you did not order, please contact us through support[at]mimetas.com and describe the products that you did receive.

Gel loading

Which ECMs / gels work in the OrganoPlate®?

The OrganoPlate® is compatible with a range of ECM gels that can transition from a liquid to a solid-state. These gels include, but are not limited to collagen-I, Matrigel®, Matrigel®-growth factor reduced BME, and HyStem gels.

How much gel do I need?

Depending on the OrganoPlate you work with and gel of choice, you need 1-2 µL of gel per tissue culture chip. Make sure to prepare a 40% excess to compensate for loss of gel due to the dead volume of the repeating pipet. For the preparation of collagen-I gel, prepare at least 100 µL total volume to ensure proper mixing of all components of the gel.

Do I need to use the complete plate at once or can it be used in parts?

It is certainly possible to use only a part of the plate. You can cover the rest of the plate with PCR film or ELISA polyester films, to ensure that they remain sterile throughout the experiments. Make sure the OrganoPlate® comes back to room temperature before seeding ECM for the second experiments.

Is it possible to coat the OrganoPlate?

The materials that make up the OrganoPlate tissue culture chips are compatible with cell culture. However, certain cell types benefit from or require the use of a coating with extracellular matrix proteins, as they also do in other culture vessels. Coating of the channels of the OrganoPlate's tissue culture chips helps to improve cell attachment, tubule formation, or biological performance of certain cell types.

How long should I incubate my gel?

The incubation time depends on your gel of choice. We advise incubating collagen-I and Matrigel® for 15-20 minutes at 37°C before adding culture medium. After incubation, the addition of medium in the adjacent medium channel will ensure the gel will not dry out. If you plan to seed tubes, add 20 µL of PBS or HBSS to the gel inlet well after gelation occurred and place in an incubator overnight before seeding the tubular cells. The PBS or HBSS should be removed from the gel inlet before you seed your cells.

What does a properly seeded gel channel look like?

After seeding your gel in the gel channel, that channel is observed as dark when you look at the bottom of the plate (you can flip the plate upside down without worrying about liquids flowing out). The adjacent medium channel(s) are still empty and observed as transparent.

When looking from the top, a thin, dark, horizontal line can be observed in the observation window, indicating your gel has filled properly. This dark line is also observed when you look at the chip under the microscope.

Why does my gel not fill the channel all the way to the end?

Improper filling of the gel channel can occur if you are working with a very viscous gel or if your gel is already partly polymerized. When working with Matrigel® or Matrigel®-like gels, thaw the gel according to the manufacturer’s instructions and make sure the gel is kept on ice continuously to prevent it from polymerizing. Using pre-cooled Eppendorf tips will also prevent this.

If your gel does not fill the gel channel properly, try increasing the seeding volume. For example, for OrganoPlate® 2-lane and 3-lane with a 400µm channel width, the range of collagen-I gel volume to seed varies between 1.5-2.1 µL. We advise starting with a seeding volume of 2 µL. If the gel does not properly fill the channel all the way, increase the seeding volume until you find the volume that works for your experiment. The Sartorius eLINE electronic repeating pipette (#735021) is very well suited for this purpose.

Why does my gel also fill the medium channel(s)?

If your gel fills both channels, you are unable to seed cells in the medium channel or to use that channel for perfusion. If you seed correctly - see our instruction manuals on how to correctly seed - overflow from the gel channel into an adjacent medium channel can be solved by lowering the gel seeding volume. The perfect seeding volume is influenced by the viscosity of the gel and the temperature in your lab. For example, for OrganoPlates® 2-lane and 3-lane with a 400µm channel width, the range of collagen-I gel volume to seed varies between 1.5-2.1 µL. We advise starting with a seeding volume of 2 µL. If overflow occurs, lower the seeding volume until you find the volume that works for your experiment. The Sartorius eLINE electronic repeating pipette (#735021) is very well suited for this purpose.

Furthermore, retain from touching the glass bottom directly. This could also lead to overflow.

What is the dark line / shadow that I observe after gel seeding?

When the gel flows into the gel channel, the PhaseGuide™ (a small rim that functions as a pressure barrier) prevents it from flowing into the adjacent medium channel(s). After filling the channel, the gel stretches partially into the next channel, which can be observed as a shadow or a dark line in the medium channel. When you see the dark line, it is a sign you’ve seeded the gel properly. Once you have added medium to the adjacent channel this dark line should have disappeared. If it remains it means there is air in the medium channel. You can read all about the physics behind the PhaseGuide™ and meniscus stretching in our publication.

What pipette should I use?

Any pipette that can dispense volumes in the 0.5-2.5 µL range can be used. However, if you are experiencing difficulties with gel loading and cell seeding, we recommend using the Sartorius eLINE® electronic pipette (Sartorius, #735021 for accurate dispensation of volumes ranging from 0.2 to 10 µL or the Eppendorf® Multipette® M4 with the Eppendorf® Biopur® 0.1 mL tip (VWR #613- 2067) for dispensation of 2 µL.

Cell culture

How can cell invasion in ECM gel be avoided?

In case of undesired cell invasion into the gel, the use of MMP inhibitors is recommended (e.g. addition of 10 μM of MMP-I inhibitor GM6001 (Abcam, ab120845) to the culture medium).

How many cells do I need?

You need 2 µL of cell suspension per tissue culture chip. The cell density of the suspension needs to be optimized for your specific culture but generally ranges from 5-30*103 cells/µL.

What cells have been tested in the OrganoPlate?

OrganoPlate® users have worked with a wide range of cells, both human and animal-derived. Primary cells, (cancer) cell lines, induced pluripotent stem cells, and organoid (derived) cells have successfully been cultured in the OrganoPlate® for among others vascular, kidney, gut, liver, CNS, and cancer models. Check our Knowledge Center for all our publications.

How do I change the medium in the OrganoPlate?

A medium change can be done by aspirating all the media from the medium inlet and outlet wells and replacing it with fresh culture medium. A mechanical single channel or multichannel aspirator can be used. Aspiration will not disturb the cells in the microfluidic channels. Place the tip(s) of the aspirator in the corner of the well while aspirating as you would do with a regular culture plate and remove all liquid. Fresh culture medium can then be added by manual or automatic pipetting into the medium inlet and outlet wells. Watch this Medium change video or read our protocol.

How often do I have to change the medium?

For most cells, we advise changing the medium 2-3 times a week (for example on Monday, Wednesday, and Friday). If your cells are highly metabolically active, you can consider changing the media more often.

Does the OrganoPlate suffer from edge effects?

No, we do not observe edge effects in the OrganoPlate®. All chips can be used for culture, including the ones near the edge of the plate.

Can I reuse my OrganoPlate after washing?

We do not recommend reusing chips in a plate, as we cannot guarantee the quality when reusing it.

Do I need to use all the chips on an OrganoPlate at the same time?

No, you can use the different chips of one plate for different experiments. When doing this make sure to cover the unused chips with adhesive film. This will prevent moisture from entering unused chips when putting the OrganoPlate in the incubator. The covered chips can then be used at a later stage. Check if the unused chips are dry under a microscope. If droplets in the microfluidic channels are observed, dry the channels by placing the plate without a lid in a flow cabinet. Make sure the plate is at room temperature before seeding.

What foil do I use to cover the unused OrganoPlate?

It is certainly possible to use only part of the plate. You can cover the rest of the plate with PCR film or ELISA polyester films, to ensure that they remain sterile throughout the experiments.

How long can the cells be cultured in OrganoPlate?

It will depend on your cell type and your seeding density. In general, we have been able to keep Caco-2 cultures for 2 weeks.

Perfusion

Do I need an OrganoFlow®?

Except for neuronal cultures, all our tissue culture models (i.e. liver, gut, kidney, vasculature, cancer, etc.) were shown to benefit strongly from medium perfusion. Perfused cultures show improved cell viability, the polarization of tubular structures, and upregulation of relevant transporters.

If you first want to try the OrganoPlate before purchasing a rocker, you can use a regular interval rocker for your first experiments. We’ve observed the formation of tubular structures of HUVECs using a regular interval rocker. However, to obtain optimal barrier formation and allow more controlled flow profiles, the OrganoFlow® is advised.

What flow speeds am I working with?

An overview of flow speed and shear stress is available upon request. Please contact support[at]mimetas.com.

Which cell types can grow without perfusion?

Neuronal cultures show optimal viability when cultured without perfusion. This includes both long-term differentiation of neural stem cells and short-term cultures of already differentiated neurons.

Is the flow bidirectional or unidirectional?

The flow is bidirectional. The OrganoPlate is placed on an OrganoFlow. A flow is created by passive liquid leveling as the medium flows from medium inlet to outlet and vice versa. This allows you to create differentiated/polarized tissues without the hassle of pumps or tubes.

What rocker settings should I use?

We observed that a 7° inclination and an 8-minute interval work well for most culture types.

Is it possible to control the flow?

Yes, you can control the flow by changing the inclination angle of the rocker as well as by changing the rocker’s interval.

Imaging

Is the OrganoPlate suitable for imaging?

Yes, the OrganoPlate is excellent for imaging purposes. The bottom of the plate is made of high optical quality 150 μm glass coverslip-thickness (170 µm) glass, allowing unparalleled optical read-outs. The OrganoPlate’s microtiter plate footprint makes it compatible with standard laboratory imaging systems.

What types of imaging can I do on the OrganoPlate?

The OrganoPlate is compatible with regular (time-lapse) phase contrast and fluorescent imaging as well as confocal imaging. The OrganoPlate is not compatible with electron microscopy.

Do I need a special microscope?

No, the OrganoPlate’s microtiter plate footprint makes it compatible with standard laboratory imaging systems.

Assays

Can I perform cell viability assays?

Yes, the OrganoPlate is compatible with various cell viability assays that employ fluorescent, luminescent, or absorbance read-outs. These assays include but are not limited to, the LIVE/DEAD® viability assay, the CellTiter-GLO™ assay, and the Alamar Blue assay.

Can I perform an immunostaining?

Yes, the OrganoPlate is ideal for immunostaining and imaging. Our immunostaining protocol can be found here. The plate is compatible with common fixation methods, such as formaldehyde and methanol fixation, and we do not observe problems with antibodies permeating the gel. If you prefer using your lab’s specific immunostaining protocol, this will very likely translate to OrganoPlate cultures as well. However, longer incubation times can be necessary compared to standard protocol (comparable to staining tissue slices).

Can I measure the integrity of my barrier tissue?

Yes, we have developed robust protocols to assess the integrity of barrier tissues such as endothelial and epithelial tubes. The protocol of our barrier integrity assay for OrganoPlate® 2-lane and 3-lane can be found here.

Can I isolate RNA and DNA from OrganoPlate cultures?

Yes, you can successfully isolate high-quality RNA or DNA from the OrganoPlate using traditional Trizol® extraction or column-based isolation kits. Depending on the nature of your culture and the amount of RNA or DNA needed, pooling the contents of several chips may be necessary to obtain sufficient material. To give you an estimate: from an endothelial tube in one chip of an OrganoPlate® 3-lane with 300 µm lane width, we typically collect 50 nanograms of RNA.

Can I sample from the OrganoPlate?

Yes, samples can be easily collected by taking the medium from the chip. This medium can be used to analyze the contents by various assays and read-outs, such as ELISA and mass spectrometry. In the OrganoPlate® 3-lane, you have access to both the apical and basal sides of the chip.

Can I isolate live cells from the OrganoPlate?

Yes, if you want to remove cells from the chips (for i.e. flow cytometry applications) you can. For instance, by trypsinizing your cells.

Cookies

‘May we use cookies?
Hi there! Thanks for visiting our website. We use cookies to keep track of our website statistics to optimize the user experience. We also use cookies for marketing purposes. You can set your preferences by selecting the options below.

Accept all
Accept selected
Decline all