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      • Layered tissues with perfused tubules in the absence of artificial membranes form the heart of our permeability and transport science. Study cell interactions, permeability, absorption, transport, and transcytosis without physical barriers.

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      • Co-culture layered & structured tissues without artificial membranes with perfect imaging, to study barrier-free cellular interactions, cell-cell signaling, and migration.

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  • About Mimetas
  • Expand your drug discovery capacity, shoulder to shoulder with our scientific team. Proprietary human disease biology in the OrganoPlate® platform. Together we make the therapeutics of tomorrow.
  • We create novel human tissue and disease models in the OrganoPlate® platform. Our experts are looking forward to developing assays according to your specifications.
  • Get robust compound data in human tissue models through our OrganoServices. With proven pheno­typic assays in the OrganoPlate® platform, we support your drug discovery and development needs.
  • We offer several services to support your drug discovery and development needs. Find the overview here.
  • Start today with 3D tissue modeling>
    Kickstart successfully your first experiments with the OrganoPlate® through our OrganoStart packages.
  • OrganoFlow® >
    Driving precisely-controlled perfusion flow in the OrganoPlate® platform

  • OrganoTEER® >
    Enabling fast, automated, and impedance-based TEER measurements in OrganoPlate®. Assay 40 tissue culture chips with a few clicks, in less than one minute.

  • Start today with 3D tissue modeling>
    Kickstart your experiments with the most sophisticated 3D tissue culture platform. Find out which training fits your 3D tissue modeling needs best.
  • OrganoPlate® is the solution for all in vitro tissue culture applications. Explore the entire OrganoPlate® family and its dedicated instruments here.

  • Thinking of using OrganoPlate for your research? Request a quote for the product(s) of your interest.

  • Layered tissues with perfused tubules in the absence of artificial membranes form the heart of our permeability and transport science. Study cell interactions, permeability, absorption, transport, and transcytosis without physical barriers.

  • Co-culture layered & structured tissues without artificial membranes with perfect imaging, to study barrier-free cellular interactions, cell-cell signaling, and migration.

  • Evaluate the effect of chemotactic triggers or cells on the migration of cells through an extracellular matrix.

  • Membrane-free microvascular formation and growth through an extracellular matrix (ECM).

  • The missing link in tissue culture: add perfusable human vasculature to your tissue models, and recreate sophisticated microenvironments with OrganoPlate® Graft.

  • OrganoPlate® enables you to study relevant 3D tissue biology by incorporating perfused tubules, co-culture, and full control over the tissue microenvironment. Find the overview of applications here.

  • Visit our Knowledge Center to get up to speed with 3D tissue culture and to learn how OrganoPlate® supports your research needs.

    Read our publications, application notes, watch our webinars, or check out the supporting protocols and brochures. All compiled for you, by our scientists.

  • Get inspired by peer-reviewed publications of our scientists, partners, and customers around the globe.

  • Get inspired by research done by our scientists, partners, and customers around the globe.

  • Giving you some food for thought. Read our blogs to learn more about 3D tissue culture, research backgrounds, developments, and its future outlook.
  • Kickstart your experiments with the most sophisticated 3D tissue culture platform. Find out which training fits your 3D tissue modeling needs best.
  • Any support questions about purchasing, products, or 3D tissue culture and analysis? Get in touch with our experts.
  • Thinking of using OrganoPlate for your research? Request a quote for the product(s) of your interest.

  • Frequently asked questions answered by our experts. Find here the answer you're looking for.
  • Learn about our mission, vision, the history of the company, and find out what we mean with MIMETAS-do.
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Blood-Brain Barrier Small Molecule Transport Assay

OrganoServicesTM are available for multiple assays to allow compound profiling and/or screening in human-relevant, complex 3D tissue models.

The blood-brain barrier (BBB) ensures a​ homeostatic environment for the central nervous system (CNS) and is essential for healthy brain functioning. A major challenge in treating CNS disorders is developing drugs that can pass through the BBB as many small-molecule pharmaceuticals are restricted from entering the brain in sufficient quantities to elicit a response. To study small molecule transport and penetration kinetics across the BBB in a high-throughput manner across the BBB , we developed a robust human brain microvascular endothelial cells (HBMEC) BBB model in the OrganoPlate® , combined with a validated small molecule transport assay.

The Blood-Brain Barrier Small Molecule Transport Assay OrganoService offers:

  • Screening of your compounds in validated, physiologically-relevant BBB model
  • Pre-QC and post-QC via barrier integrity measurements
  • Screens are run in sets of 6 compounds in one concentration
  • 4 technical replicates and 4 cell-free controls per compound
  • Digoxin and Propranolol used as control compounds (May be switched with your compounds)
  • Basal and apical samples shared
  • Extend the compound screen with more plates, in case a larger screen is desired

An example of an application that can be performed using this OrganoServiceTM is to evaluate BBB permeability of potential samll-molecule compounds.


About Perfused Tubules OrganoPlate 3-lane 64

 

Service details

About Blood-Brain Barrier Small Molecule Transport Assay

Kickstart drug permeability studies in a physiologically​ relevant 3D human BBB model​
Set up and perform your BBB transport assay in a perfusable human brain microvascular endothelial model​

Complement your BBB transport studies with additional toxicity studies​
Assess the toxicity of your compounds and their effect on the HBMEC barrier with our OrganoService BBB toxicity assay​

Robust and reproducible assay​
Automated workflow ensures consistent performance and data quality​

Sampling & data reporting in one go​
Receive basal and apical samples, as well as post-QC data​

​Membrane- and cell-free transport​
Perform transport measurements without the obstruction of artificial membranes and cells

Service flyer Contact us for more info

Want to know more?


Get up to speed with 3D tissue culture and learn how OrganoPlate® supports your research needs.

Knowledge Center Contact us

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