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Colon Caco-2 Barrier Integrity

OrganoServicesTM are available for multiple assays to allow compound profiling and/or screening in human-relevant, complex 3D tissue models.

When developing new drugs, it is critical to understand the permeability, absorption, and transport mechanisms in relevant biological systems. An important prerequisite to study this is the epithelial barrier function. To assess the barrier function of epithelia in vitro electrical measurement of the impedance is the golden standard. We developed a barrier integrity assay using Caco‑2 intestinal tubules cultured in the OrganoPlate® together with the recently introduced OrganoTEER®, that measures the transepithelial electrical resistance (TEER) of perfused epithelial tubes.

OrganoService Colon Caco-2 Barrier Integrity offers:

  • Screening of your compounds in our developed Caco-2 intestinal model
  • Screening of up to 16 compounds in one concentration per OrganoPlate
  • 2 Technical replicates and 3 control samples
  • Option to extend the compound screen with more plates, in case a larger screen is desired
  • Raw and normalized TEER values in a clear data report

Some examples of applications that can be performed using this OrganoServiceTM are toxicity tests of chemicals or therapeutic candidates and evaluation of food ingredients for gastrointestinal health effects.


About Perfused Tubules OrganoTEER

 

Service details

About OrganoService
Colon Caco-2 Barrier Integrity

 

Kickstart the screening of your compounds in 3D tissue cultures
Easy access to our OrganoPlate® platform and high-quality 3D tissue cultures

Time-lapse measurements
High sensitivity of drug-induced time-lapse measurements

Data reporting in one go
Receive a clear data report and raw data sheets

Consistent data quality
Fully automated data acquisition and extraction ensures consistent data quality

Compiled by our scientists
Service is developed from the internal R&D knowledge base

Using OrganoTEER for better representation of data
Barrier integrity data obtained with the OrganoTEER® results in more representative data for the gut barrier then assays using a membrane


Service flyerContact us for more info

Get inspired


Webinar
In Vitro Intestine Permeability Models in 4 Days

App Note
Comparison of the OrganoPlate® and the Transwell® Platform for In Vitro Intestinal Permeability Assays

Publication
2021 High throughput transepithelial electrical resistance (TEER) measurements on perfused membrane-free epithelia., Nicolas A. et al.

Blog
Enhanced patient-specific intestine-on-a-chip model for disease modeling and drug discovery

All OrganoServices

Want to know more?


Get up to speed with 3D tissue culture and learn how OrganoPlate® supports your research needs.

Knowledge Center Contact us

Living innovation

What customers say
'
Our exciting collaboration enables our research and early development group to apply state-of-the-art organ-on-a-chip technology in the modeling of IBD and HBV. These models have the potential to improve our understanding of disease biology and transform

James Sabry, Global Head
Roche Pharma Partnering

What customers say
'
"The OrganoReady Colon Caco-2, combined with the OrganoTEER, have become our gold standard in vitro model for the screening of intestinal barrier disruptors at SIAF. "

Cezmi Akdis
Director SIAF
University Zurich

What customers say
'
The OrganoPlate Caco-2 model is for us, at Merck, the most suitable platform offering at the same time scalability, speed, robustness, ease of handling, with low compound needs.

Philip Hewitt
Head of Early Investigative Toxicology
Merck KGaA

The 'I' in MIMETAS
Chiwan Chiang
Marketing Specialist
'
A day without
laughter is a day wasted.
Read more
What customers say
'
Mimetas' tumor infiltration platform demonstrated a therapeutic effect of our compound on immune cell recruitment: we’re very excited by these proof of concept data

Scientist
From a top pharma

The 'I' in MIMETAS
Jos Joore
CEO
'
We want to
empower research on complex tissue biology.
Read more
What customers say
'
Often you have to compromise it’s either the throughput or it’s the complexity of the model. Getting both in the same platform… no other platform can do that!

Scientist
From a top pharma

The 'I' in MIMETAS
Nienke Wevers
Senior Scientist
'
Nothing ventured, nothing gained.
Read more
What customers say
'
We were able to measure cell barrier permeabilities in a lung-on-a-chip model demonstrating superior sensitivity and translatability than any other lung cell model we tried before. It was also very easy to scale up our data generation workflow.

Scientist
From a top commercial organization

The 'I' in MIMETAS
Henriëtte Lanz
Director of Model Development
'
It should be
mandatory to
socialize with colleagues.
Read more
What customers say
'
Organoplates opened avenues that were before considered unreachable

Laura Perin, PhD
Children’s Hospital Los Angeles

The 'I' in MIMETAS
Bas Trietsch
Chief Technology Officer
'
We try to help
each other
as a team.
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The 'I' in MIMETAS
Jacquelien Ten Dam
Chief Financial Officer
'
It always seems impossible until it’s done.
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The 'I' in MIMETAS
Paul Vulto
CEO
'
Leveraging who you are, is the key to making a difference!
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The 'I' in MIMETAS
Tina Thakkar
Associate Director of Sales
'
It is not really
work if you are
having fun.
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The 'I' in MIMETAS
Liis Rannamäe
Laboratory Technician
'
Can be summarized in three words - engaging, playful, and innovative.
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The 'I' in MIMETAS
Mieke Doornbos
Vice President Sales & Marketing
'
Celebrate
good times.
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The 'I' in MIMETAS
Frederik Schavemaker
Product Design Engineer
'
Pioneering, bringing the best solutions
for scientific
discovery.
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The 'I' in Mimetas
Remko van Vught
Vice President Business Development
'
Making
a difference
together.
Read more
The 'I' in MIMETAS
Lenie van den Broek
Director Biology Discovery
'
Dynamic and innovative - each day holds a surprise.
Read more
The 'I' in Mimetas
Arnaud Nicolas
Associate Director TechCore
'
Own your flaws.
Read more
Expert Oncology Discussion
'
New Roundtable Discussion



Therapeutic Developments and Innovative Strategies in Oncology: An Expert Discussion
Watch it here

Press Release
'
MIMETAS and Astellas Enter into Strategic Partnership on Immuno-oncology

The partnership comprises utilizing MIMETAS’ comprehensive tumor models to support the next generation of immuno-oncological therapies.

Read the full article here

Grow.Learn.Discover. Event
'
A Decade of Breakthroughs in Human Tissue Modeling

Looking back at a Celebration of a Decade of Breakthroughs from our online anniversary event this past February, 9th, 2023.

Access the on-demand recordings here

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