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  • We create novel human tissue and disease models in the OrganoPlate® platform. Our experts are looking forward to developing assays according to your specifications.
  • Expand your drug discovery capacity, shoulder to shoulder with our scientific team. Proprietary human disease biology in the OrganoPlate® platform. Together we make the therapeutics of tomorrow.
  • We offer several services to support your drug discovery and development needs. Find the overview here.
  • Layered tissues with perfused tubules in the absence of artificial membranes form the heart of our permeability and transport science. Study cell interactions, permeability, absorption, transport, and transcytosis without physical barriers.

  • Co-culture layered & structured tissues without artificial membranes with perfect imaging, to study barrier-free cellular interactions, cell-cell signaling, and migration.

  • Evaluate the effect of chemotactic triggers or cells on the migration of cells through an extracellular matrix.

  • Membrane-free microvascular formation and growth through an extracellular matrix (ECM).

  • The missing link in tissue culture: add perfusable human vasculature to your tissue models, and recreate sophisticated microenvironments with OrganoPlate® Graft.

  • OrganoPlate® enables you to study relevant 3D tissue biology by incorporating perfused tubules, co-culture, and full control over the tissue microenvironment. Find the overview of applications here.

  • Visit our Knowledge Center to get up to speed with 3D tissue culture and to learn how OrganoPlate® supports your research needs.

    Read our publications, application notes, watch our webinars, or check out the supporting protocols and brochures. All compiled for you, by our scientists.

  • Get inspired by peer-reviewed publications of our scientists, partners, and customers around the globe.

  • Get inspired by research done by our scientists, partners, and customers around the globe.

  • Giving you some food for thought. Read our blogs to learn more about 3D tissue culture, research backgrounds, developments, and its future outlook.
  • Kickstart your experiments with the most sophisticated 3D tissue culture platform. Find out which training fits your 3D tissue modeling needs best.
  • Any support questions about purchasing, products, or 3D tissue culture and analysis? Get in touch with our experts.
  • Thinking of using OrganoPlate for your research? Request a quote for the product(s) of your interest.

  • Frequently asked questions answered by our experts. Find here the answer you're looking for.
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EN

TEER measurements on perfused membrane-free epithelia

Leiden, April 16, 2021 - Scientists from MIMETAS, Sciospec and Leiden University introduce an instrument that measures TransEpithelial Electrical Resistance (TEER) of perfused epithelial tubes in the microfluidic OrganoPlate® platform. The research is recently published in the Journal Lab on a Chip.

Electrical measurement of the impedance is the gold standard for assessing the barrier function of epithelia in vitro. This so-called TEER measurement correlates electrical properties of an epithelial layer with biological aspects such as cell layer confluency and thickness, tight junction formation, and morphology. Assessment of epithelial barrier function is critically important for studying healthy and diseased biological models.

TEER properties were investigated of the intestinal carcinoma cell line Caco-2 as well as the quasi-immortalized renal proximal tubule cell line RPTEC. Our measurements confirmed increasing TEER values with tubular growth over time and decreasing values in response to toxic compounds.

To our best knowledge, this is the first instrument of its kind that allows routine TEER studies in perfused Organ-on-a-Chip systems without interference by artificial filter membranes. By not using a porous membrane, our setup allows to directly measure the TEER of the cell layer only, avoiding skewing the results by membrane characteristics such as pore size and density, as well as compensation for ECM coating and/or deposition.

The instrument is revolutionary in terms of its throughput, ease-of-use, and capability to interrogate epithelia under flow and without interference of porous membranes. We anticipate its routine use in both academic research and pharmaceutical development.

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