Vascular models in Nature Sci Rep | Mimetas

Vascular models in Nature Sci Rep

Vascular models in Nature Sci Rep

In Nature Scientific Reports: 96 Perfusable blood vessels to study vascular permeability

A tubular, perfused vascular model in the OrganoPlate(r)

Current in vitro models to test the barrier function of vasculature are based on flat, two-dimensional monolayers. These monolayers do not have the tubular morphology of vasculature found in vivo and lack important cues from the cellular microenvironment, such as interaction with extracellular matrix (ECM) and exposure to flow. To increase the physiological relevance of in vitro vascular models, it is crucial to implement these aspects to better mimic the native, 3D vascular architecture.

Throughput combined with physiological relevance

In Van Duinen et al., we present a robust, high-throughput method to culture 3D perfused microvasculature in the OrganoPlate®. The paper also describes a quantitative, real-time barrier integrity assay to assess barrier function of vascular models. Culture conditions were optimized to obtain mature, functional microvessels in only 7 days, that were viable for over 60 days. The microvessels were impermeable for 150 kDa dextran, but are permeable for 20 kDa dextran, thus mimicking the characteristics of in vivo vasculature. Also, dose-dependent effects of VEGF, TNFα and several cytokines were observed, further substantiating the physiological relevance of these models.

The throughput and robustness of this model and assays will allow researchers in vascular biology to make an easy transition from 2D to 3D culture methods.

Link to the publication