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  • Technology
  • Services
    • Drug Development Partnerships
      • Expand your drug discovery capacity, shoulder to shoulder with our scientific team. Proprietary human disease biology in the OrganoPlate® platform. Together we make the therapeutics of tomorrow.
    • Custom Model & Assay Development
      • We create novel human tissue and disease models in the OrganoPlate® platform. Our experts are looking forward to developing assays according to your specifications.
    • Compound profiling & Screening
      • Get robust compound data in human tissue models through our services. With proven pheno­typic assays in the OrganoPlate® platform, we support your drug discovery and development needs.
    • All Services
      • We offer several services to support your drug discovery and development needs. Find the overview here.
  • Products
  • Applications
    • Perfused Tubules

      • Layered tissues with perfused tubules in the absence of artificial membranes form the heart of our permeability and transport science. Study cell interactions, permeability, absorption, transport, and transcytosis without physical barriers.

    • Cell-Cell Interactions

      • Co-culture layered & structured tissues without artificial membranes with perfect imaging, to study barrier-free cellular interactions, cell-cell signaling, and migration.

    • 3D Cell Migration

      • Evaluate the effect of chemotactic triggers or cells on the migration of cells through an extracellular matrix.

    • Angiogenesis

      • Membrane-free microvascular formation and growth through an extracellular matrix (ECM).

    • Vascularization

      • The missing link in tissue culture: add perfusable human vasculature to your tissue models, and recreate sophisticated microenvironments with OrganoPlate® Graft.

    • All Applications

      • OrganoPlate® enables you to study relevant 3D tissue biology by incorporating perfused tubules, co-culture, and full control over the tissue microenvironment. Find the overview of applications here.

  • Resources & Support
    • Knowledge Center

      • Visit our Knowledge Center to get up to speed with 3D tissue culture and to learn how OrganoPlate® supports your research needs.

        Read our publications, application notes, watch our webinars, or check out the supporting protocols and brochures. All compiled for you, by our scientists.

    • Publications

      • Get inspired by peer-reviewed publications of our scientists, partners, and customers around the globe.

    • Case Studies

      • Get inspired by research done by our scientists, partners, and customers around the globe.

    • Blogs
      • Giving you some food for thought. Read our blogs to learn more about 3D tissue culture, research backgrounds, developments, and its future outlook.
    • Workshops & Trainings
      • Kickstart your experiments with the most sophisticated 3D tissue culture platform. Find out which training fits your 3D tissue modeling needs best.
    • Technical Support
      • Any support questions about purchasing, products, or 3D tissue culture and analysis? Get in touch with our experts.
    • Request a quote

      • Thinking of using OrganoPlate for your research? Request a quote for the product(s) of your interest.

    • FAQ
      • Frequently asked questions answered by our experts. Find here the answer you're looking for.
  • Knowledge Center
  • About Mimetas
  • Expand your drug discovery capacity, shoulder to shoulder with our scientific team. Proprietary human disease biology in the OrganoPlate® platform. Together we make the therapeutics of tomorrow.
  • We create novel human tissue and disease models in the OrganoPlate® platform. Our experts are looking forward to developing assays according to your specifications.
  • Get robust compound data in human tissue models through our services. With proven pheno­typic assays in the OrganoPlate® platform, we support your drug discovery and development needs.
  • We offer several services to support your drug discovery and development needs. Find the overview here.
  • Start today with 3D tissue modeling
    Kickstart your first experiments with the OrganoPlate® through our OrganoStart packages.

  • OrganoFlow®
    Driving precisely-controlled perfusion flow in the OrganoPlate® platform

  • OrganoTEER®
    Enabling fast, automated, and impedance-based TEER measurements in OrganoPlate®. Assay 40 or 64 tissue culture chips with a few clicks, in less than two minutes.

  • Start today with 3D tissue modeling
    Kickstart your experiments with the most sophisticated 3D tissue culture platform. Find out which training fits your 3D tissue modeling needs best.

  • OrganoPlate® is the solution for all in vitro tissue culture applications. Explore the entire OrganoPlate® family and its dedicated instruments here.

  • Thinking of using OrganoPlate for your research? Request a quote for the product(s) of your interest.

  • Layered tissues with perfused tubules in the absence of artificial membranes form the heart of our permeability and transport science. Study cell interactions, permeability, absorption, transport, and transcytosis without physical barriers.

  • Co-culture layered & structured tissues without artificial membranes with perfect imaging, to study barrier-free cellular interactions, cell-cell signaling, and migration.

  • Evaluate the effect of chemotactic triggers or cells on the migration of cells through an extracellular matrix.

  • Membrane-free microvascular formation and growth through an extracellular matrix (ECM).

  • The missing link in tissue culture: add perfusable human vasculature to your tissue models, and recreate sophisticated microenvironments with OrganoPlate® Graft.

  • OrganoPlate® enables you to study relevant 3D tissue biology by incorporating perfused tubules, co-culture, and full control over the tissue microenvironment. Find the overview of applications here.

  • Visit our Knowledge Center to get up to speed with 3D tissue culture and to learn how OrganoPlate® supports your research needs.

    Read our publications, application notes, watch our webinars, or check out the supporting protocols and brochures. All compiled for you, by our scientists.

  • Get inspired by peer-reviewed publications of our scientists, partners, and customers around the globe.

  • Get inspired by research done by our scientists, partners, and customers around the globe.

  • Giving you some food for thought. Read our blogs to learn more about 3D tissue culture, research backgrounds, developments, and its future outlook.
  • Kickstart your experiments with the most sophisticated 3D tissue culture platform. Find out which training fits your 3D tissue modeling needs best.
  • Any support questions about purchasing, products, or 3D tissue culture and analysis? Get in touch with our experts.

  • Thinking of using OrganoPlate for your research? Request a quote for the product(s) of your interest.

  • Frequently asked questions answered by our experts. Find here the answer you're looking for.
  • Learn about our mission, vision, the history of the company, and find out what we mean with MIMETAS-do.
EN

Vascularization

The missing link in tissue culture: add perfusable human vasculature to your tissue models, and recreate sophisticated microenvironments
with OrganoPlate® Graft.

Why vascularization?

One of the key issues in organoid research is that it is impossible to add perfused human vasculature. Functional blood vessels are a vital connecting structure inside the body (‘in vivo’) and this aspect is lacking in conventional in vitro cell culture techniques. The vascularization of human tissue can therefore only be achieved in vivo by transplanting these tissues into mice that have been manipulated to lack an immune system. This to avoid rejection of the implant, denoted patient-derived xenograft (PDX).

The PDX process is extremely inefficient, costly, time-consuming, and can only be performed in limited numbers since it is missing throughput. Also, the human tissues are compromised by the ingrowth of murine tissue, including mouse vasculature, and thus not capturing the in vivo human situation. Facing these challenges and knowing that the entire PDX process is under high ethical pressure, we see a great unmet need for cell culture techniques that add human vasculature to in vitro cultured 3D tissues.

For this reason, we have expanded the OrganoPlate® family with the OrganoPlate® Graft. The first in vitro cell culture technique that allows vascularization of 3D tissues such as spheroids, organoids, and tumors.

Features

  • In vitro grafting of target tissues on a vascular bed
  • Study interaction between perfused vasculature & target tissues
  • Spheroids, organoids, tissue fragments
  • Patient-derived tissue grafting in vitro: an alternative to PDX mice


Assays

  • Immunostaining of endothelial cell markers and markers of grafted tissue, and 3D reconstructions
  • Perfusion assay, using fluorescent dyes
  • Tissue dependent assays, e.g. albumin assay
  • Graft tissue processing, immunohistochemical and histopathological characterization

About OrganoPlate® Graft

OrganoPlate® Graft: the missing link in tissue culture

OrganoPlate® Graft is a variation of the OrganoPlate 2-lane and 3-lane series and incorporates MIMETAS' unique technology to grow functional microvessels to create a microvascular bed. Tissue placed onto this bed is connected to the system of human blood vessels, achieving in vitro vascularization. This facilitates perfusion of the tissue and allows drug administration through the vasculature.

The OrganoPlate® Graft platform offers the possibility to vascularize a plethora of different tissue types on a standard SBS plate format and is compatible with a high-throughput workflow. It offers functionality that is not yet seen in the field of in vitro tissue culture modeling and has the potential to enhance current and future in vitro vascularization research.

Our Technology

Related

In a nutshell

OrganoPlate® Graft is developed for grafting tissues to a vascular bed. Its open-top design makes it possible to receive target tissues for studying interaction with a vascular bed.

Download the PDF to learn in a few simplified steps how the platform works.

Download PDF

Products


Product Overview

Get inspired

Case Study
Robust angiogenesis inhibition assay in collaboration with Leiden University Medical Center (LUMC)

App Note
The OrganoPlate® Graft: in vitro vascularization of human tissues

App Note
Perfused 3D angiogenic sprouting in a high-throughput in vitro platform

 

Want to know more?


Get up to speed with 3D tissue culture and learn how OrganoPlate® supports your research needs.

Knowledge Center Contact us

What customers say
'
Our exciting collaboration enables our research and early development group to apply state-of-the-art organ-on-a-chip technology in the modeling of IBD and HBV. These models have the potential to improve our understanding of disease biology and transform

James Sabry, Global Head
Roche Pharma Partnering

The 'I' in Mimetas
Chiwan Chiang
Field Application Specialist
'
A day without
laughter is a day wasted.
Read more
What customers say
'
Mimetas' tumor infiltration platform demonstrated a therapeutic effect of our compound on immune cell recruitment: we’re very excited by these proof of concept data

Scientist
From a top pharma

The 'I' in Mimetas
Jos Joore
CEO
'
We want to
empower research on complex tissue biology.
Read more
What customers say
'
Often you have to compromise it’s either the throughput or it’s the complexity of the model. Getting both in the same platform… no other platform can do that!

Scientist
From a top pharma

The 'I' in Mimetas
Nienke Wevers
Senior Scientist
'
Nothing ventured, nothing gained.
Read more
What customers say
'
We were able to measure cell barrier permeabilities in a lung-on-a-chip model demonstrating superior sensitivity and translatability than any other lung cell model we tried before. It was also very easy to scale up our data generation workflow.

Scientist
From a top commercial organization

The 'I' in Mimetas
Henriëtte Lanz
Director of Model Development
'
It should be
mandatory to
socialize with colleagues.
Read more
What customers say
'
Organoplates opened avenues that were before considered unreachable

Laura Perin, PhD
Children’s Hospital Los Angeles

The 'I' in Mimetas
Bas Trietsch
Chief Technology Officer
'
We try to help
each other
as a team.
Read more
What customers say
'
The OrganoReady model has been very useful for fast screening of novel gene therapy related technologies in our company. The membrane-free fluidics is a high throughput screening tool to monitor a transfer of antibodies through the blood brain barrier.

Svetlana Pasteuning
VectorY B.V., Netherlands

The 'I' in Mimetas
Jacquelien Ten Dam
Chief Financial Officer
'
It always seems impossible until it’s done.
Read more
The 'I' in Mimetas
Paul Vulto
CEO
'
Leveraging who you are, is the key to making a difference!
Read more
Expert Oncology Discussion
'
New Roundtable Discussion



Therapeutic Developments and Innovative Strategies in Oncology: An Expert Discussion
Watch it here

Press Release
'
MIMETAS and Astellas Enter into Strategic Partnership on Immuno-oncology

The partnership comprises utilizing MIMETAS’ comprehensive tumor models to support the next generation of immuno-oncological therapies.

Read the full article here

Grow.Learn.Discover. Event
'
A Decade of Breakthroughs in Human Tissue Modeling

Join us on February, 9th 2023, for our online anniversary event.

Register here

Press Release
'
MIMETAS elected most innovative company of the Netherlands


MIMETAS is the winner of
the Dutch Innovation Award 2022

Read the full article here

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