• We offer tailored partnerships to support and transform drug discovery.

    Every collaboration is a bespoke arrangement, meticulously designed to discover new compounds and targets in patient relevant disease models.

  • MIMETAS offers flexible fee-for-service solutions for therapy prioritization, optimization, and de-risking of compounds.

    As an extension of your team, MIMETAS provides the expertise and resources needed for effective research and development.

  • Our OrganoReady program is made for you when you need optimized assays for investigational toxicology.

    We guarantee OrganoReady performance according to specifications, in a fast and convenient sales transaction with a clear fee structure.

  • Giving you some food for thought. Read our blogs to learn more about 3D tissue culture, research backgrounds, developments, and its future outlook.
  • Get inspired by research done by our scientists, partners, and customers around the globe.

  • Learn about our mission, vision, the history of the company, and find out what we mean with MIMETAS-do.
open menu icon close menu icon
EN

Vascularization

The missing link in tissue culture: add perfusable human vasculature to your tissue models, and recreate sophisticated microenvironments
with OrganoPlate® Graft.

Why vascularization?

One of the key issues in organoid research is that it is impossible to add perfused human vasculature. Functional blood vessels are a vital connecting structure inside the body (‘in vivo’) and this aspect is lacking in conventional in vitro cell culture techniques. The vascularization of human tissue can therefore only be achieved in vivo by transplanting these tissues into mice that have been manipulated to lack an immune system. This to avoid rejection of the implant, denoted patient-derived xenograft (PDX).

The PDX process is extremely inefficient, costly, time-consuming, and can only be performed in limited numbers since it is missing throughput. Also, the human tissues are compromised by the ingrowth of murine tissue, including mouse vasculature, and thus not capturing the in vivo human situation. Facing these challenges and knowing that the entire PDX process is under high ethical pressure, we see a great unmet need for cell culture techniques that add human vasculature to in vitro cultured 3D tissues.

For this reason, we have expanded the OrganoPlate® family with the OrganoPlate® Graft. The first in vitro cell culture technique that allows vascularization of 3D tissues such as spheroids, organoids, and tumors.

Features

  • In vitro grafting of target tissues on a vascular bed
  • Study interaction between perfused vasculature & target tissues
  • Spheroids, organoids, tissue fragments
  • Patient-derived tissue grafting in vitro: an alternative to PDX mice


Assays

  • Immunostaining of endothelial cell markers and markers of grafted tissue, and 3D reconstructions
  • Perfusion assay, using fluorescent dyes
  • Tissue dependent assays, e.g. albumin assay
  • Graft tissue processing, immunohistochemical and histopathological characterization

Speak With an Expert

OrganoPlate® Graft: the missing link in tissue culture

OrganoPlate® Graft is a variation of the OrganoPlate 2-lane and 3-lane series and incorporates MIMETAS' unique technology to grow functional microvessels to create a microvascular bed. Tissue placed onto this bed is connected to the system of human blood vessels, achieving in vitro vascularization. This facilitates perfusion of the tissue and allows drug administration through the vasculature.

The OrganoPlate® Graft platform offers the possibility to vascularize a plethora of different tissue types on a standard SBS plate format and is compatible with a high-throughput workflow. It offers functionality that is not yet seen in the field of in vitro tissue culture modeling and has the potential to enhance current and future in vitro vascularization research.

Our Technology

Want to know more?


Get up to speed with 3D tissue culture and learn how OrganoPlate® supports your research needs.

Knowledge Center Contact us

Cookies

May we use cookies?
Hi there! Thanks for visiting our website. We use cookies to keep track of our website statistics to optimize the user experience. We also use cookies for marketing purposes. You can set your preferences by selecting the options below. Terms of Use & Privacy Policy
Accept all
Accept selected
Decline all