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Angiogenesis

Membrane-free microvascular formation and growth
through an extracellular matrix (ECM).

Why angiogenesis?

The loss of vascular integrity plays a rate-limiting role in the onset and progression of diseases, such as atherosclerosis and cancer, and conditions such as chronic inflammation and ischemia. Therefore, detailed knowledge of the mechanisms of microvascular loss or the formation of novel vascular structures such as those generated by angiogenesis is of major importance.

Angiogenic sprouting is the growth of new blood vessels from pre-existing vessels, which is orchestrated by cues from within the cellular microenvironment, such as biochemical gradients and perfusion. Many of these cues are missing in current in vitro models of angiogenic sprouting. We have optimized the OrganoPlate® platform for growing perfusable 3D angiogenic sprouts, including tip-stalk cell hierarchy and anastomosis. The platform integrates both perfusion and the generation of stable biomolecular gradients and demonstrates its potential to study more physiologically relevant angiogenic sprouting and microvascular stabilization.

All Applications

Features

  • Perfused 3D microvasculature
  • Stable capillary structure for 4+ days
  • Tip-stalk cell hierarchy & anastomosis
  • Investigate microvascular damage, tumor angiogenesis, fibrosis

 

Assays

  • Sprout visualization through calcein-AM labeling
  • Sprout quantification (based on fluorescent images)
  • Perfusion assay, using fluorescent dyes
  • Expression of adherens and tight junction markers (immunostaining)
  • Expression of transporters (RNA isolation and PCR, or immunostaining)

How to grow 3D angiogenesis models?


OrganoPlate allows you to study angiogenesis in 3D. In this video, we explain to you in a few steps how we develop 3D angiogenesis models at MIMETAS. Watch it now and learn how we can support your research.

Our Technology

Want to know more?


Get up to speed with 3D tissue culture and learn how OrganoPlate® supports your research needs.

Knowledge Center Contact us

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