The loss of vascular integrity plays a rate-limiting role in the onset and progression of diseases, such as atherosclerosis and cancer, and conditions such as chronic inflammation and ischemia. Therefore, detailed knowledge of the mechanisms of microvascular loss or the formation of novel vascular structures such as those generated by angiogenesis is of major importance.
Angiogenic sprouting is the growth of new blood vessels from pre-existing vessels, which is orchestrated by cues from within the cellular microenvironment, such as biochemical gradients and perfusion. Many of these cues are missing in current in vitro models of angiogenic sprouting. We have optimized the OrganoPlate® platform for growing perfusable 3D angiogenic sprouts, including tip-stalk cell hierarchy and anastomosis. The platform integrates both perfusion and the generation of stable biomolecular gradients and demonstrates its potential to study more physiologically relevant angiogenic sprouting and microvascular stabilization.
OrganoPlate allows you to study angiogenesis in 3D. This timelapse shows a culture of human umbilical vein endothelial cells (HUVECs) forming angiogenic sprouts in the OrganoPlate® within 4 days.