OrganoServicesTM are available for multiple assays to allow compound profiling and/or screening in human-relevant, complex 3D tissue models.
Angiogenesis is crucial in the onset and progression of many diseases, including cancer, macular degeneration, Alzheimer’s disease, vascular fibrosis, inflammation, and kidney failure associated with diabetes. This entails that targeting angiogenesis has a vast therapeutic value in a wide range of diseases. To study angiogenesis in vitro we developed an angiogenic sprouting assay with Human Umbilical Vein Endothelial Cells (HUVECs) cultured as micro-vessels in the OrganoPlate®. A study with a protein kinase inhibitor library of 1546 compounds was performed as a proof of concept.
OrganoService Angiogenesis HUVEC offers:
- Screening of your compounds in our developed Angiogenesis HUVEC model
- Screening of up to 24 compounds in one concentration per OrganoPlate
- 2 Technical replicates and 3 control samples
- Option to extend the compound screen with more plates, in case a larger screen is desired
- Phenotypic sprouting assay 48h after compound exposure, sprout length is assessed by nuclei migration distance
- Raw and normalized nuclei distance values in a clear data report
An example of an application that can be performed using this OrganoServiceTM is to evaluate potential anti-angiogenic compounds for sprouting inhibition.