Pancreatic Ductal Adenocarcinoma (PDAC) is one of the most lethal cancers due to a high chemoresistance and poor vascularization, which results in an ineffective systemic therapy. PDAC is characterized by a high intratumoral pressure, which is not captured by current 2D and 3D in vitro models.
Together with Leiden Academic Centre for Drug Research (LACDR) we developed a 3D microfluidic interstitial flow model to capture the intratumoral pressure in PDAC. In this study, pancreatic cancer organoids were cultured in an ECM gel in the OrganoPlate® 3-lane 40. Flow was applied parallel and orthogonal to the organoids in the gel, in order to compare lateral flow with interstitial flow through the tissue.
This project received funding from the European Union’s Seventh Framework Programme for research, technological development, and demonstration under grant agreement no 602783. The full study was published in Int. J. Mol. Sci. 2019, 20, 4647.
We developed a 3D cell culture modality for studying intratissue pressure and flow. By subjecting the PDAC cell line to interstitial flow, we showed that:
The model exhibits more predictive capabilities than conventional 2D cell culture, is less time-consuming, and more scalable and accessible than animal models. This increase in microphysiological relevance might support improved efficiency in the drug development pipeline.