Chat with us, powered by LiveChat
  • Technology
  • Products
  • Services
    • Compound profiling & Screening
      • Get robust compound data in human tissue models through our OrganoServices. With proven pheno­typic assays in the OrganoPlate® platform, we support your drug discovery and development needs.
    • Custom Model & Assay Development
      • We create novel human tissue and disease models in the OrganoPlate® platform. Our experts are looking forward to developing assays according to your specifications.
    • Drug Development Partnerships
      • Expand your drug discovery capacity, shoulder to shoulder with our scientific team. Proprietary human disease biology in the OrganoPlate® platform. Together we make the therapeutics of tomorrow.
    • All Services
      • We offer several services to support your drug discovery and development needs. Find the overview here.
  • Applications
    • Perfused Tubules
      • Layered tissues with perfused tubules in the absence of artificial membranes form the heart of our permeability and transport science. Study cell interactions, permeability, absorption, transport, and transcytosis without physical barriers.

    • Cell-Cell Interactions
      • Co-culture layered & structured tissues without artificial membranes with perfect imaging, to study barrier-free cellular interactions, cell-cell signaling, and migration.

    • 3D Cell Migration
      • Evaluate the effect of chemotactic triggers or cells on the migration of cells through an extracellular matrix.

    • Angiogenesis
      • Membrane-free microvascular formation and growth through an extracellular matrix (ECM).

    • Vascularization
      • The missing link in tissue culture: add perfusable human vasculature to your tissue models, and recreate sophisticated microenvironments with OrganoPlate® Graft.

    • All Applications
      • OrganoPlate® enables you to study relevant 3D tissue biology by incorporating perfused tubules, co-culture, and full control over the tissue microenvironment. Find the overview of applications here.

  • Resources & Support
    • Knowledge Center
      • Visit our Knowledge Center to get up to speed with 3D tissue culture and to learn how OrganoPlate® supports your research needs.

        Read our publications, application notes, watch our webinars, or check out the supporting protocols and brochures. All compiled for you, by our scientists.

    • Publications
      • Get inspired by peer-reviewed publications of our scientists, partners, and customers around the globe.

    • Case Studies
      • Get inspired by research done by our scientists, partners, and customers around the globe.

    • Blogs
      • Giving you some food for thought. Read our blogs to learn more about 3D tissue culture, research backgrounds, developments, and its future outlook.
    • Workshops & Trainings
      • Kickstart your experiments with the most sophisticated 3D tissue culture platform. Find out which training fits your 3D tissue modeling needs best.
    • Technical Support
      • Any support questions about purchasing, products, or 3D tissue culture and analysis? Get in touch with our experts.
    • Request a quote
      • Thinking of using OrganoPlate for your research? Request a quote for the product(s) of your interest.

    • FAQ
      • Frequently asked questions answered by our experts. Find here the answer you're looking for.
  • About Mimetas
  • Start today with 3D tissue modeling>
    Kickstart successfully your first experiments with the OrganoPlate® through our OrganoStart packages.
  • OrganoFlow® >
    Driving precisely-controlled perfusion flow in the OrganoPlate® platform

  • OrganoTEER® >
    Enabling fast, automated, and impedance-based TEER measurements in OrganoPlate®. Assay 40 tissue culture chips with a few clicks, in less than one minute.

  • Start today with 3D tissue modeling>
    Kickstart your experiments with the most sophisticated 3D tissue culture platform. Find out which training fits your 3D tissue modeling needs best.
  • OrganoPlate® is the solution for all in vitro tissue culture applications. Explore the entire OrganoPlate® family and its dedicated instruments here.

  • Thinking of using OrganoPlate for your research? Request a quote for the product(s) of your interest.

  • Get robust compound data in human tissue models through our OrganoServices. With proven pheno­typic assays in the OrganoPlate® platform, we support your drug discovery and development needs.
  • We create novel human tissue and disease models in the OrganoPlate® platform. Our experts are looking forward to developing assays according to your specifications.
  • Expand your drug discovery capacity, shoulder to shoulder with our scientific team. Proprietary human disease biology in the OrganoPlate® platform. Together we make the therapeutics of tomorrow.
  • We offer several services to support your drug discovery and development needs. Find the overview here.
  • Layered tissues with perfused tubules in the absence of artificial membranes form the heart of our permeability and transport science. Study cell interactions, permeability, absorption, transport, and transcytosis without physical barriers.

  • Co-culture layered & structured tissues without artificial membranes with perfect imaging, to study barrier-free cellular interactions, cell-cell signaling, and migration.

  • Evaluate the effect of chemotactic triggers or cells on the migration of cells through an extracellular matrix.

  • Membrane-free microvascular formation and growth through an extracellular matrix (ECM).

  • The missing link in tissue culture: add perfusable human vasculature to your tissue models, and recreate sophisticated microenvironments with OrganoPlate® Graft.

  • OrganoPlate® enables you to study relevant 3D tissue biology by incorporating perfused tubules, co-culture, and full control over the tissue microenvironment. Find the overview of applications here.

  • Visit our Knowledge Center to get up to speed with 3D tissue culture and to learn how OrganoPlate® supports your research needs.

    Read our publications, application notes, watch our webinars, or check out the supporting protocols and brochures. All compiled for you, by our scientists.

  • Get inspired by peer-reviewed publications of our scientists, partners, and customers around the globe.

  • Get inspired by research done by our scientists, partners, and customers around the globe.

  • Giving you some food for thought. Read our blogs to learn more about 3D tissue culture, research backgrounds, developments, and its future outlook.
  • Kickstart your experiments with the most sophisticated 3D tissue culture platform. Find out which training fits your 3D tissue modeling needs best.
  • Any support questions about purchasing, products, or 3D tissue culture and analysis? Get in touch with our experts.
  • Thinking of using OrganoPlate for your research? Request a quote for the product(s) of your interest.

  • Frequently asked questions answered by our experts. Find here the answer you're looking for.
  • Learn about our mission, vision, the history of the company, and find out what we mean with MIMETAS-do.
EN

Case study: 3D immune cell migration towards a tumor microenvironment

Evaluating the effect of compounds on transendothelial migration of immune cells towards a tumor microenvironment.

About the research

Recruitment and transendothelial migration of T cells is a crucial aspect of immune system functioning and can result in the onset or propagation of disease if aberrant. Most research studying transendothelial migration of T cells is performed in mouse models or in vitro using migration assays across monolayers of cells that do not recapitulate the complex human in vivo situation. For this reason, we developed an endothelial vessel perfused with T cells in a membrane-free and tubular manner against a collagen hydrogel. Tumor cells or chemokines can be added as a chemotactic trigger. Such 3D co-culture models allow studying recruitment and infiltration of T cells in healthy or diseased environments and could support the development of novel (immuno)therapeutics.

The use of a patients’ own immune system proved to be a promising avenue for combatting cancer although therapeutic efficacy varies significantly depending on the tumor type and between patients. Novel models that manage to better recapitulate the behavior of T cells in vivo could potentially spur the development and improvement of these immune therapies. This knowledge led us to the development of a 3D tissue culture model where we could better understand this behavior. The model compromises an endothelial vessel perfused with T cells that can be used to study the migration of the T cells towards a tumor compartment in a high-throughput manner.

Custom model & assay

We have used the OrganoPlate® 3-lane 40 to grow blood vessels perfused with T cells. This type of OrganoPlate features 40 independent tissue culture chips with 3 adjacent channels per chip. We added Collagen I extracellular matrix (ECM) gel to the middle channel and seeded endothelial cells against the ECM gel in the top channel. After adhesion of the endothelial cells, we started perfusion of the culture to form an endothelial tubular structure. Perfusion was performed by placing the OrganoPlate on the OrganoFlow®.

Following T cell migration over time

To eventually mimic the tumor microenvironment, we seeded tumor cells in or against the gel in the bottom perfusion channel. As a final step, we perfused the endothelial vessel with fluorescently stained T cells to follow the migration of T cells towards the tumor compartment for 48 hours. By following this migration over time, we observed that both unstimulated and stimulated T cells migrated towards the tumor cells.

The migration of T cells could be stimulated by adding an additional chemotactic trigger (chemokine) to the medium in the bottom channel or inhibited by pre-treatment of the endothelial tube with a compound.

Key results

  • 3D immune cell migration through an extracellular matrix towards a tumor compartment
  • T cell extravasation from a blood vessel
  • Allows excellent imaging, cell tracking & quantification
  • Study the effects of compounds on immune cell migration

About Cell Migration

Keep me updated

Want to know more?


Get up to speed with 3D tissue culture and learn how OrganoPlate® supports your research needs.

Knowledge Center Contact us

Cookies

‘May we use cookies?
Hi there! Thanks for visiting our website. We use cookies to keep track of our website statistics to optimize the user experience. We also use cookies for marketing purposes. You can set your preferences by selecting the options below.

Accept all
Accept selected
Decline all