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  • About Mimetas
  • Supporting up to 96 tissue models on a single plate. Its unique PhaseGuide™ technology enables cells to interact and migrate freely between the two channels of each chip.

  • Supporting up to 40 tissue models on a single plate. Its unique PhaseGuide™ technology enables cells to interact and migrate freely between the three channels of each chip.
  • Supporting up to 64 tissue models on a single plate. Specially designed for automated workflows. Its unique PhaseGuide™ technology enables cells to interact and migrate freely between the three channels of each chip.
  • The first in vitro tissue culture platform that allows co-culture of spheroids, organoids, and tumors with a perfused microvascular bed and vascularization of 3D tissues.

  • Offering 38 ready-to-use Caco-2 tubules for drug exposure, transport, and permeability studies.
  • Driving precisely-controlled perfusion flow in the OrganoPlate® platform
  • Enabling fast, automated, and impedance-based TEER measurements in OrganoPlate®. Assay 40 tissue culture chips with a few clicks, in less than one minute.
  • Kickstart your experiments with the most sophisticated 3D tissue culture platform. Find out which training fits your 3D tissue modeling needs best.
  • OrganoPlate® is the solution for all in vitro tissue culture applications. Explore the entire OrganoPlate® family and its dedicated instruments here.

  • Get robust compound data in human tissue models. With proven pheno­typic assays in the OrganoPlate® platform, we support your drug discovery and development needs.
  • We create novel human tissue and disease models in the OrganoPlate® platform. Our experts are looking forward to developing assays according to your specifications.
  • Expand your drug discovery capacity, shoulder to shoulder with our scientific team. Proprietary human disease biology in the OrganoPlate® platform. Together we make the therapeutics of tomorrow.
  • We offer several services to support your drug discovery and development needs. Find the overview here.
  • Layered tissues with perfused tubules in the absence of artificial membranes form the heart of our permeability and transport science. Study cell interactions, permeability, absorption, transport, and transcytosis without physical barriers.

  • Co-culture layered & structured tissues without artificial membranes with perfect imaging, to study barrier-free cellular interactions, cell-cell signaling, and migration.

  • Evaluate the effect of chemotactic triggers or cells on the migration of cells through an extracellular matrix.

  • Membrane-free microvascular formation and growth through an extracellular matrix (ECM).

  • The missing link in tissue culture: add perfusable human vasculature to your tissue models, and recreate sophisticated microenvironments with OrganoPlate® Graft.

  • OrganoPlate® enables you to study relevant 3D tissue biology by incorporating perfused tubules, co-culture, and full control over the tissue microenvironment. Find the overview of applications here.

  • Visit our Knowledge Center to get up to speed with 3D tissue culture and to learn how OrganoPlate® supports your research needs.

    Read our publications, application notes, watch our webinars, or check out the supporting protocols and brochures. All compiled for you, by our scientists.

  • Get inspired by peer-reviewed publications of our scientists, partners, and customers around the globe.

  • Get inspired by research done by our scientists, partners, and customers around the globe.

  • Giving you some food for thought. Read our blogs to learn more about 3D tissue culture, research backgrounds, developments, and its future outlook.
  • Kickstart your experiments with the most sophisticated 3D tissue culture platform. Find out which training fits your 3D tissue modeling needs best.
  • Any support questions about purchasing, products, or 3D tissue culture and analysis? Get in touch with our experts.
  • Thinking of using OrganoPlate for your research? Request a quote for the product(s) of your interest.

  • Frequently asked questions answered by our experts. Find here the answer you're looking for.
  • Learn about our mission, vision, the history of the company, and find out what we mean with MIMETAS-do.
ENGLISH

Case study: Antibody transcytosis across the blood-brain barrier (BBB)

Studying antibody transcytosis in an unique 3D BBB-on-a-chip model.

About the research

The brain vasculature is made up of specialized endothelial cells that form a tight blood-brain barrier (BBB). This barrier prevents many drugs as well as large biological molecules such as antibodies, from passively diffusing into the brain. For this reason, specialized techniques need to be developed to improve drug targeting to the brain. One of these techniques is receptor mediated transcytosis (RMT) of antibodies. This entails the design of a therapeutic antibody that binds to a receptor present on the brain endothelial cells, such as the transferrin receptor (TfR), thus improving its targeting to the brain. After binding to the TfR on the apical side of the brain endothelial cells, the antibody is transcytosed and subsequently released on the basolateral side, i.e. the brain side.

In vitro models of the blood-brain barrier are of great value for evaluating, for example, the efficiency of antibody transcytosis across the BBB and improve the treatment of neurodegenerative diseases.

Custom model & assay

We developed an in vitro BBB-on-a-chip model comprising human brain endothelial cells, astrocytes, and pericytes. The endothelial cells formed a functional barrier that is able to retain fluorescent dyes and antibodies, and that prevent antibodies from leaking out of the model through passive mechanisms.

Perfusion with a target antibody

The model was perfused with a target antibody, designed to cross the BBB via targeting of the TfR. Next to this, we perfused the model with a control antibody that doesn’t bind to human cells and, therefore is not supposed to cross.

We found an approximately twofold higher passage rate of the target antibody compared to the control antibody, indicating that this model is indeed suitable for the evaluation of BBB shuttle technologies.

Key results

  • Unique 3D and membrane-free BBB-on-a-chip model compromising human brain endothelial cells, astrocytes, and pericytes
  • Formation of a functional barrier able to retain fluorescent dyes and antibodies
  • First BBB in vitro model that includes flow and can study barrier function and antibody transcytosis in high-throughput

About Cell-Cell Interactions

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