OrganoReady® Colon Caco-2
OrganoReady® Colon Caco-2 is a ready-to-use in vitro intestinal model comprised of perfusable, leak-tight Caco-2 tubules. These tubules feature apical and basolateral access without the use of artificial membranes and develop a functional barrier enabling direct compound exposure, sampling, and reliable high-throughput intestinal barrier assessment and gastrointestinal toxicity (GI) screening.
Specifications
Each OrganoReady Caco-2 comes pre-seeded with 40 or 64 ready-to-use Caco-2 tubules grown against validated Collagen-I with stable barriers.
Cell Type: Caco-2
Format: OrganoPlate® 3-lane 64 or 40
Configuration: Caco-2 tubules against Collagen-I
Access: Apical and basolateral, membrane free
Recovery Time: Functional barrier re-forms within a few days post-arrival; no additional seeding or preparation required
Assessment of intestinal barrier integrity
Gastrointestinal toxicity screening
Modeling intestinal inflammation
Applications
The OrganoReady Colon Caco-2 model offers a standardized in vitro gut model that supports consistent and reproducible assay conditions across applications. All applications benefit from our OrganoTEER system for barrier function measurement.
Validation
The OrganoReady Colon Caco-2 model demonstrates characteristics of a polarized and differentiated intestinal epithelium. The model features asymmetric localization of key transporters and markers, reflecting functional apical–basolateral polarity.
The Caco-2 model shows lumen formation and confirms the 3D architecture of the epithelial tubules
The Caco-2 tubules show differentiated phenotype (ZO-1, Occludin and Acetylated Tubulin)
The Caco-2 tubules show Apical (Ezrin and MRP) and Basolateral (Glut2) polarized epithelium
Enterotoxin Screening in Caco-2 Tubules
A high-throughput gut-on-chip platform to study the epithelial responses to enterotoxins: This publication demonstrates how a Caco-2 tubule model enables high-throughput, multiparametric assessment of enterotoxin-induced epithelial damage, offering improved sensitivity and translatability over conventional intestinal models.
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How to Access Our OrganoReady® Colon Caco-2
Services
MIMETAS provides tailored, fee-for-service solutions to support therapy selection, optimization, and risk assessment. Using the OrganoReady® Caco-2 model, we offer scalable screening assays for inflammatory and compound safety studies .
Shipped Product
The OrganoReady® Caco-2 is offered as a ready-to-use model shipped directly to your lab, complete with culture media and optimized protocols. After a short recovery, the perfusable tubules are assay-ready, ideal for intestinal barrier integrity and gastrointestinal toxicity studies.