App Note: 3D Immune Cell Migration
Immune cell migration is an essential process for many biological phenomena such as early embryonic development and immune cell response. During inflammation, the recruitment and migration of T cells through the vascular system towards the site of inflammation is a crucial component.In this app note, we demonstrate the development of an in vitro assay utilizing the OrganoPlate® platform to study T cell dynamics in combination with a blood vessel structure under perfused conditions, as well as the high-content imaging used to observe the migration of cells at various time points.Benefits of the model:Create complex cell migration assays that are easily scalable and compatible for high-content imagingPerform quantitative assessment of immune cell dynamics, generating high throughput readouts such as cell numbers and cell migration distance
App Note: 3D Immune Cell Migration
App Note: 3D Immune Cell Migration
A perfused 3D immune model: The OrganoPlate® platform enables real-time analysis of T cell transendothelial migration under inflammatory conditions by integrating a vessel structure with extracellular matrix and high-content imaging for quantification.
App Note: 3D Immune Cell Migration
A perfused 3D immune model: The OrganoPlate® platform enables real-time analysis of T cell transendothelial migration under inflammatory conditions by integrating a vessel structure with extracellular matrix and high-content imaging for quantification.
App Note: 3D Immune Cell Migration
A perfused 3D immune model: The OrganoPlate® platform enables real-time analysis of T cell transendothelial migration under inflammatory conditions by integrating a vessel structure with extracellular matrix and high-content imaging for quantification.
App Note: 3D Immune Cell Migration
A perfused 3D immune model: The OrganoPlate® platform enables real-time analysis of T cell transendothelial migration under inflammatory conditions by integrating a vessel structure with extracellular matrix and high-content imaging for quantification.
App Note: 3D Immune Cell Migration
A perfused 3D immune model: The OrganoPlate® platform enables real-time analysis of T cell transendothelial migration under inflammatory conditions by integrating a vessel structure with extracellular matrix and high-content imaging for quantification.
App Note: 3D Immune Cell Migration
A perfused 3D immune model: The OrganoPlate® platform enables real-time analysis of T cell transendothelial migration under inflammatory conditions by integrating a vessel structure with extracellular matrix and high-content imaging for quantification.
App Note: 3D Immune Cell Migration
A perfused 3D immune model: The OrganoPlate® platform enables real-time analysis of T cell transendothelial migration under inflammatory conditions by integrating a vessel structure with extracellular matrix and high-content imaging for quantification.
App Note: 3D Immune Cell Migration
A perfused 3D immune model: The OrganoPlate® platform enables real-time analysis of T cell transendothelial migration under inflammatory conditions by integrating a vessel structure with extracellular matrix and high-content imaging for quantification.