Novel in vitro model to better recapitulate the behavior of T cells in vivo
The use of a patients’ own immune system is a promising avenue to combat cancer, even though therapeutic efficacy varies significantly depending on the tumor type and between patients. Novel models that manage to better recapitulate the behavior of T cells in vivo could spur the development and improvement of these immune therapies.
In this poster, we present a novel in vitro 3D T cell migration model comprising an endothelial tubule perfused with T cells that can be used to study their migration in healthy and inflammatory conditions in a high-throughput manner.
Using the OrganoPlate® platform, directional T-cell migration across endothelial vessel walls into adjacent tissue can be tracked and quantified. Also, migration can be modulated by the addition of a wide range of triggers. Furthermore, the platform enables the incorporation of other tissues or cells, such as tumor cells. The development of the model in a high-throughput 3D tissue culture platform could support future screening purposes.
Download the poster here