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      • Supporting up to 96 tissue models on a single plate. Its unique PhaseGuide™ technology enables cells to interact and migrate freely between the two channels of each chip.

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      • Supporting up to 40 tissue models on a single plate. Its unique PhaseGuide™ technology enables cells to interact and migrate freely between the three channels of each chip.
    • OrganoPlate® 3-lane 64
      • Supporting up to 64 tissue models on a single plate. Specially designed for automated workflows. Its unique PhaseGuide™ technology enables cells to interact and migrate freely between the three channels of each chip.
    • OrganoPlate® Graft
      • The first in vitro tissue culture platform that allows co-culture of spheroids, organoids, and tumors with a perfused microvascular bed and vascularization of 3D tissues.

    • OrganoReady® Caco-2
      • Offering 38 ready-to-use Caco-2 tubules for drug exposure, transport, and permeability studies.
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      • Driving precisely-controlled perfusion flow in the OrganoPlate® platform
    • OrganoTEER®
      • Enabling fast, automated, and impedance-based TEER measurements in OrganoPlate®. Assay 40 tissue culture chips with a few clicks, in less than one minute.
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      • Kickstart your experiments with the most sophisticated 3D tissue culture platform. Find out which training fits your 3D tissue modeling needs best.
    • All Products
      • OrganoPlate® is the solution for all in vitro tissue culture applications. Explore the entire OrganoPlate® family and its dedicated instruments here.

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      • Get robust compound data in human tissue models. With proven pheno­typic assays in the OrganoPlate® platform, we support your drug discovery and development needs.
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      • We create novel human tissue and disease models in the OrganoPlate® platform. Our experts are looking forward to developing assays according to your specifications.
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  • Applications
    • Perfused Tubules
      • Layered tissues with perfused tubules in the absence of artificial membranes form the heart of our permeability and transport science. Study cell interactions, permeability, absorption, transport, and transcytosis without physical barriers.

    • Cell-Cell Interactions
      • Co-culture layered & structured tissues without artificial membranes with perfect imaging, to study barrier-free cellular interactions, cell-cell signaling, and migration.

    • 3D Cell Migration
      • Evaluate the effect of chemotactic triggers or cells on the migration of cells through an extracellular matrix.

    • Angiogenesis
      • Membrane-free microvascular formation and growth through an extracellular matrix (ECM).

    • Vascularization
      • The missing link in tissue culture: add perfusable human vasculature to your tissue models, and recreate sophisticated microenvironments with OrganoPlate® Graft.

    • All Applications
      • OrganoPlate® enables you to study relevant 3D tissue biology by incorporating perfused tubules, co-culture, and full control over the tissue microenvironment. Find the overview of applications here.

  • Resources & Support
    • Knowledge Center
      • Visit our Knowledge Center to get up to speed with 3D tissue culture and to learn how OrganoPlate® supports your research needs.

        Read our publications, application notes, watch our webinars, or check out the supporting protocols and brochures. All compiled for you, by our scientists.

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    • Workshops & Trainings
      • Kickstart your experiments with the most sophisticated 3D tissue culture platform. Find out which training fits your 3D tissue modeling needs best.
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  • About Mimetas
  • Supporting up to 96 tissue models on a single plate. Its unique PhaseGuide™ technology enables cells to interact and migrate freely between the two channels of each chip.

  • Supporting up to 40 tissue models on a single plate. Its unique PhaseGuide™ technology enables cells to interact and migrate freely between the three channels of each chip.
  • Supporting up to 64 tissue models on a single plate. Specially designed for automated workflows. Its unique PhaseGuide™ technology enables cells to interact and migrate freely between the three channels of each chip.
  • The first in vitro tissue culture platform that allows co-culture of spheroids, organoids, and tumors with a perfused microvascular bed and vascularization of 3D tissues.

  • Offering 38 ready-to-use Caco-2 tubules for drug exposure, transport, and permeability studies.
  • Driving precisely-controlled perfusion flow in the OrganoPlate® platform
  • Enabling fast, automated, and impedance-based TEER measurements in OrganoPlate®. Assay 40 tissue culture chips with a few clicks, in less than one minute.
  • Kickstart your experiments with the most sophisticated 3D tissue culture platform. Find out which training fits your 3D tissue modeling needs best.
  • OrganoPlate® is the solution for all in vitro tissue culture applications. Explore the entire OrganoPlate® family and its dedicated instruments here.

  • Get robust compound data in human tissue models. With proven pheno­typic assays in the OrganoPlate® platform, we support your drug discovery and development needs.
  • We create novel human tissue and disease models in the OrganoPlate® platform. Our experts are looking forward to developing assays according to your specifications.
  • Expand your drug discovery capacity, shoulder to shoulder with our scientific team. Proprietary human disease biology in the OrganoPlate® platform. Together we make the therapeutics of tomorrow.
  • We offer several services to support your drug discovery and development needs. Find the overview here.
  • Layered tissues with perfused tubules in the absence of artificial membranes form the heart of our permeability and transport science. Study cell interactions, permeability, absorption, transport, and transcytosis without physical barriers.

  • Co-culture layered & structured tissues without artificial membranes with perfect imaging, to study barrier-free cellular interactions, cell-cell signaling, and migration.

  • Evaluate the effect of chemotactic triggers or cells on the migration of cells through an extracellular matrix.

  • Membrane-free microvascular formation and growth through an extracellular matrix (ECM).

  • The missing link in tissue culture: add perfusable human vasculature to your tissue models, and recreate sophisticated microenvironments with OrganoPlate® Graft.

  • OrganoPlate® enables you to study relevant 3D tissue biology by incorporating perfused tubules, co-culture, and full control over the tissue microenvironment. Find the overview of applications here.

  • Visit our Knowledge Center to get up to speed with 3D tissue culture and to learn how OrganoPlate® supports your research needs.

    Read our publications, application notes, watch our webinars, or check out the supporting protocols and brochures. All compiled for you, by our scientists.

  • Get inspired by peer-reviewed publications of our scientists, partners, and customers around the globe.

  • Get inspired by research done by our scientists, partners, and customers around the globe.

  • Giving you some food for thought. Read our blogs to learn more about 3D tissue culture, research backgrounds, developments, and its future outlook.
  • Kickstart your experiments with the most sophisticated 3D tissue culture platform. Find out which training fits your 3D tissue modeling needs best.
  • Any support questions about purchasing, products, or 3D tissue culture and analysis? Get in touch with our experts.
  • Thinking of using OrganoPlate for your research? Request a quote for the product(s) of your interest.

  • Frequently asked questions answered by our experts. Find here the answer you're looking for.
  • Learn about our mission, vision, the history of the company, and find out what we mean with MIMETAS-do.
ENGLISH

Robust and versatile renal tubule-on-a-chip for nephrotoxicity assessment

Detecting nephrotoxic liabilities with Nephroscreen

Leiden, March 22, 2021 - Scientists from MIMETAS, Roche Innovation Center, Pfizer, GlaxoSmithKline (GSK), Utrecht Institute for Pharmaceutical Sciences, University of Applied Sciences Northwestern Switzerland, and the Swiss Centre for Applied Human Toxicology, developed a high throughput, 3D microfluidic platform (Nephroscreen) to detect drug-induced nephrotoxicity. The research is recently published in the journal Current Opinion in Toxicology.

Proximal tubule epithelial cells (PTEC) are the main driver of renal transport and secretion of xenobiotics, making them susceptible to drug-induced kidney injury (DIKI). Cell-based assays are a meaningful alternative to animal testing to detect nephrotoxicity and contribute to the 3Rs (Refine, Reduce, Replace animal experimentation). Here we report on a high throughput, 3D microfluidic platform (Nephroscreen) to detect drug-induced nephrotoxicity. Toxicologically relevant parameters were used to assess cell viability, functional epithelial barrier integrity, and interactions with specific transporters (P-glycoprotein: P-gp and multidrug resistance-associated protein 2/4: MRP2/4).

Nephroscreen allowed the combination of a variety of read-outs including imaging, extracellularly released markers, intracellular markers, and functional assays. Nephroscreen is compatible with automated pipetting, proved to be amenable to long-term experiments (at least eleven days) and was easily transferred between laboratories. The compelling data originates from several published reports on the development and implementation of this platform to detect nephrotoxicity and drug-transporter interactions. The reports demonstrate that Nephroscreen could be used to detect the nephrotoxic liabilities of the tested compounds. Future directions should include additional test compounds and thorough validation of its performance.

This project was supported under the Crack-it challenge 15 (Nephrotube) project no. 37497-25920, an initiative of the National Center for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs).

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