The development of drugs for the treatment of intestinal inflammatory conditions remains difficult due to the lack of reliable, robust, and high throughput in vitro models. Current models lack physiological relevance because they do not recapitulate key aspects of the intestine. Organ-on-a-chip technology can be utilized as a solution to this issue by modeling tissues in 3D to more closely resemble in vivo conditions. In this webinar, Linda Gijzen will discuss the development of a complex co-cultured organ-on-a-chip model to study the inflamed intestine.
The model contains multiple intestinal and immune cell types to increase physiological relevance. With the use of this model, biological modularity was achieved, representing key aspects of the inflamed intestine. This platform is compatible with high throughput, robust assays in which complexity that can be tailored to serve all preclinical stages in the drug discovery process.
In this webinar, you will learn:
- How to develop an intestine-on-a-chip model in the OrganoPlate®
- How to measure intestinal inflammation using high throughput TEER measurements
- How to verify the physiological relevance of your 3D tissue model
Linda Gijzen, Scientific Project Lead at MIMETAS
Linda completed her Master’s degree in biomedical sciences, specializing in toxicology and human pathobiology in 2016 and is currently working as a Scientific Project Lead at MIMETAS. In this role, she is involved in the development and characterization of 3D human based microfluidic models of the kidney, intestine, heart, and immune oncology. Besides her position as Scientific Project Lead, she is doing a Ph.D. at Mimetas and the University of Utrecht focusing on microfluidic disease models of the kidney and intestine.
- Gijzen, L., Marescotti, D., Raineri, E. et al. SLAS TECHNOLOGY: Translating Life Sciences Innovation (2020) 25.6: 585-597. https://doi.org/10.1177/2472630320924999
- Weller, A., Hansen, M. B., Marie, R. et al. Bioengineering systems for therapeutic and in vitro platforms (2022) 16648714: 189. https://doi.org/10.3389/fbioe.2022.965200
- Kosim, K., Schilt, I., Lanz, H. L. et al. Organ-on-a-Chip: Methods and Protocols (2022) 87-105. https://doi.org/10.1007/978-1-0716-1693-2_6
- Naumovska, E., Aalderink, G., Wong Valencia, C. et al. International journal of molecular sciences (2020) 21.14: 4964. https://doi.org/10.3390/ijms21144964