Studying angiogenesis in a manner that is representative of the in vivo situation can be a challenging task. Crucial elements in modeling angiogenesis are the formation of 3-dimensional sprouts having a lumen and clearly defined tip and stalk cells. Other important aspects include the presence of an extracellular matrix and the perfusion of blood vessels.
What if there was a way to study angiogenesis in vitro in a way that would allow you to model all these aspects of angiogenesis in a easily and quickly way?
- You could not only study the onset of angiogenesis, but also key aspects that occur during the maturation phase.
- You would be able to access the lumina of your angiogenic sprouts and perfuse them too.
- Would you like to apply a stable gradient of growth factors to direct the formation of capillaries?
You can do that too! Think about all the possibilities that would open up, if you had such a system. In this webinar, you will learn about a robust microfluidic platform (OrganoPlate®) that will allow you to do all of the above. The OrganoPlate® platform is already successfully being used by the top 10 pharma as well as by hundreds of academic researchers worldwide. Unlike most microfluidic devices used today, the OrganoPlate® does not make use of any tubings nor pumps. As a matter of fact, it looks and handles exactly like a standard 384-wells plate. This means that it is compatible with all your equipment.
In this webinar, you will learn how to work with collagen gel, form a blood vessel, applying a perfusion flow, and creating a gradient of angiogenic factors so that you can study the onset of angiogenesis, as well as key aspects that occur during the maturation phase.
What you will learn
- Why current in vitro angiogenesis models have limitations
- How to develop perfusable angiogenic sprouts and assess their functionality
- How to use this 3D angiogenic model to mimic the tumor microenvironment
What others have said about the OrganoPlate®
“With the MIMETAS OrganoPlate, we developed a 3D brain-on-a-chip model that made it easy to do high-throughput and high-content imaging to evaluate the acute toxicity of neurotoxins. The OrganoPlate allowed the study of membrane-free vascular formation in a dynamic environment and further provided reliable and repeatable experiments with strong imaging capability.” — Dr. Yeoheung Yun, Associate Professor at North Carolina A&T State University
“Often you have to compromise: it’s either the throughput or it’s the complexity of the model. Getting both in the same platform…no other platform can do that!” – Senior Scientist from top pharma
- van Duinen V, Stam W, Borgdorff V, et al. J Vis Exp. (2019) 153:10. https://doi.org/10.3791/59678
- van Duinen, V., Zhu, D., Ramakers, C. et al. Angiogenesis (2019) 22:157–165. https://doi.org/10.1007/s10456-018-9647-0
- van Duinen, V., van den Heuvel, A., Trietsch, S.J. et al. Sci Rep (2017) 7:18071. https://doi.org/10.1038/s41598-017-14716-y