Are you frustrated with your animal models to study nephrotoxicity? Or is your 2D cell culture model keeping you up at night? You know that having reliable results from nephrotoxicity studies can make or break the introduction of a new compound to the market. The stakes are too high. These studies cannot be left to poor models that fail to translate to in vivo or models that have limited physiological relevance.
In this webinar, you will learn about a robust in vitro system, the OrganoPlate®, to perform in vitro studies on 3D perfused proximal tubules. You will learn how to develop a model of fully polarized renal proximal tubular epithelial cells expressing active transporters needed for your studies. You will discover how to obtain reliable, consistent, and physiologically relevant results quickly using human cell lines in advanced 3D cell culture models.
The OrganoPlate® platform is already successfully being used by the top 10 pharma as well as by hundreds of academic researchers worldwide.
What you will learn
- Limitations of current kidney proximal tubule models and how to overcome them
- How to build a leak-tight 3D in vitro kidney proximal tubule model with fully polarized renal proximal tubular epithelial cells thanks to flow and co-cultures
- How to obtain consistent and reproducible data
- How to easily assess proximal tubules’ permeability and integrity to gauge your model leak-tightness
What others have said about the OrganoPlate®
“With the MIMETAS OrganoPlate, we developed a 3D brain-on-a-chip model that made easy to do high-throughput and high-content imaging to evaluate the acute toxicity of neurotoxins. OrganoPlate allowed the study of membrane-free vascular formation in a dynamic environment and further provided reliable and repeatable experiments with strong imagining capability.” — Dr. Yeoheung Yun, Associate Professor at North Carolina A&T State University
“Often you have to compromise: it’s either the throughput or it’s the complexity of the model. Getting both in the same platform…no other platforms can do that!” – Senior Scientist from top pharma
- Lai, R.E. & Masereeuw, R. AAPS J (2018) 20: 98. https://doi.org/10.1208/s12248-018-0258-x
- Vormann, M.K., Gijzen, L., Hutter, S. et al. AAPS J (2018) 20: 90. https://doi.org/10.1208/s12248-018-0248-z
- Vriend, J., Nieskens, T.T.G., Vormann, M.K. et al. AAPS J (2018) 20: 87. https://doi.org/10.1208/s12248-018-0247-0
- Suter-Dick, L., Mauch, L., Ramp, D. et al. AAPS J (2018) 20: 86. https://doi.org/10.1208/s12248-018-0245-2