Chat with us, powered by LiveChat
  • Technology
  • Services
    • Drug Development Partnerships
      • Expand your drug discovery capacity, shoulder to shoulder with our scientific team. Proprietary human disease biology in the OrganoPlate® platform. Together we make the therapeutics of tomorrow.
    • Custom Model & Assay Development
      • We create novel human tissue and disease models in the OrganoPlate® platform. Our experts are looking forward to developing assays according to your specifications.
    • Compound profiling & Screening
      • Get robust compound data in human tissue models through our services. With proven pheno­typic assays in the OrganoPlate® platform, we support your drug discovery and development needs.
    • All Services
      • We offer several services to support your drug discovery and development needs. Find the overview here.
  • Products
  • Applications
    • Perfused Tubules
      • Layered tissues with perfused tubules in the absence of artificial membranes form the heart of our permeability and transport science. Study cell interactions, permeability, absorption, transport, and transcytosis without physical barriers.

    • Cell-Cell Interactions
      • Co-culture layered & structured tissues without artificial membranes with perfect imaging, to study barrier-free cellular interactions, cell-cell signaling, and migration.

    • 3D Cell Migration
      • Evaluate the effect of chemotactic triggers or cells on the migration of cells through an extracellular matrix.

    • Angiogenesis
      • Membrane-free microvascular formation and growth through an extracellular matrix (ECM).

    • Vascularization
      • The missing link in tissue culture: add perfusable human vasculature to your tissue models, and recreate sophisticated microenvironments with OrganoPlate® Graft.

    • All Applications
      • OrganoPlate® enables you to study relevant 3D tissue biology by incorporating perfused tubules, co-culture, and full control over the tissue microenvironment. Find the overview of applications here.

  • Resources & Support
    • Knowledge Center
      • Visit our Knowledge Center to get up to speed with 3D tissue culture and to learn how OrganoPlate® supports your research needs.

        Read our publications, application notes, watch our webinars, or check out the supporting protocols and brochures. All compiled for you, by our scientists.

    • Publications
      • Get inspired by peer-reviewed publications of our scientists, partners, and customers around the globe.

    • Case Studies
      • Get inspired by research done by our scientists, partners, and customers around the globe.

    • Blogs
      • Giving you some food for thought. Read our blogs to learn more about 3D tissue culture, research backgrounds, developments, and its future outlook.
    • Workshops & Trainings
      • Kickstart your experiments with the most sophisticated 3D tissue culture platform. Find out which training fits your 3D tissue modeling needs best.
    • Technical Support
      • Any support questions about purchasing, products, or 3D tissue culture and analysis? Get in touch with our experts.
    • Request a quote
      • Thinking of using OrganoPlate for your research? Request a quote for the product(s) of your interest.

    • FAQ
      • Frequently asked questions answered by our experts. Find here the answer you're looking for.
  • Knowledge Center
  • About Mimetas
  • Expand your drug discovery capacity, shoulder to shoulder with our scientific team. Proprietary human disease biology in the OrganoPlate® platform. Together we make the therapeutics of tomorrow.
  • We create novel human tissue and disease models in the OrganoPlate® platform. Our experts are looking forward to developing assays according to your specifications.
  • Get robust compound data in human tissue models through our services. With proven pheno­typic assays in the OrganoPlate® platform, we support your drug discovery and development needs.
  • We offer several services to support your drug discovery and development needs. Find the overview here.
  • Start today with 3D tissue modeling
    Kickstart your first experiments with the OrganoPlate® through our OrganoStart packages.
  • OrganoFlow®
    Driving precisely-controlled perfusion flow in the OrganoPlate® platform

  • OrganoTEER®
    Enabling fast, automated, and impedance-based TEER measurements in OrganoPlate®. Assay 40 or 64 tissue culture chips with a few clicks, in less than two minutes.

  • Start today with 3D tissue modeling
    Kickstart your experiments with the most sophisticated 3D tissue culture platform. Find out which training fits your 3D tissue modeling needs best.
  • OrganoPlate® is the solution for all in vitro tissue culture applications. Explore the entire OrganoPlate® family and its dedicated instruments here.

  • Thinking of using OrganoPlate for your research? Request a quote for the product(s) of your interest.

  • Layered tissues with perfused tubules in the absence of artificial membranes form the heart of our permeability and transport science. Study cell interactions, permeability, absorption, transport, and transcytosis without physical barriers.

  • Co-culture layered & structured tissues without artificial membranes with perfect imaging, to study barrier-free cellular interactions, cell-cell signaling, and migration.

  • Evaluate the effect of chemotactic triggers or cells on the migration of cells through an extracellular matrix.

  • Membrane-free microvascular formation and growth through an extracellular matrix (ECM).

  • The missing link in tissue culture: add perfusable human vasculature to your tissue models, and recreate sophisticated microenvironments with OrganoPlate® Graft.

  • OrganoPlate® enables you to study relevant 3D tissue biology by incorporating perfused tubules, co-culture, and full control over the tissue microenvironment. Find the overview of applications here.

  • Visit our Knowledge Center to get up to speed with 3D tissue culture and to learn how OrganoPlate® supports your research needs.

    Read our publications, application notes, watch our webinars, or check out the supporting protocols and brochures. All compiled for you, by our scientists.

  • Get inspired by peer-reviewed publications of our scientists, partners, and customers around the globe.

  • Get inspired by research done by our scientists, partners, and customers around the globe.

  • Giving you some food for thought. Read our blogs to learn more about 3D tissue culture, research backgrounds, developments, and its future outlook.
  • Kickstart your experiments with the most sophisticated 3D tissue culture platform. Find out which training fits your 3D tissue modeling needs best.
  • Any support questions about purchasing, products, or 3D tissue culture and analysis? Get in touch with our experts.
  • Thinking of using OrganoPlate for your research? Request a quote for the product(s) of your interest.

  • Frequently asked questions answered by our experts. Find here the answer you're looking for.
  • Learn about our mission, vision, the history of the company, and find out what we mean with MIMETAS-do.
EN

A microfluidic-based PDAC organoid system reveals the impact of hypoxia in response to treatment

Leiden, February 3, 2023 – MIMETAS scientists established a 3D Pancreatic Ductal Adenocarcinoma (PDAC) model, enabling the study of cancer treatment response in PDAC organoids.

Complex and physiologically relevant tumor models are critical for effective drug discovery and development. This is particularly relevant for tumors which lack effective treatments such as PDAC. Characterized by a high-density stroma that makes up to 90% of the tumor volume, PDAC comprises a variety of cells, mainly including cancer-associated fibroblasts, and pancreatic stellate cells (PSCs) as well as extracellular matrix components. In addition to having dense stroma, another hallmark of PDAC is extreme hypoxia. It is therefore essential to recapitulate these oxygen levels seen in PDAC, as well as relevant cell types and other integral conditions, when developing PDAC models.

In this study, MIMETAS researchers developed PDAC organoid-based models that included stromal cells as well as low oxygen tension that emulate that of the tumor core. Using the OrganoPlate® 2-lane, a versatile 3D microfluidic platform, they cultured PDAC-PSCs co-cultures, under normoxic and hypoxic conditions. Here, they found phenotypic and transcriptional changes associated with hypoxia. In addition, they investigated different chemotherapeutic treatment responses of PDAC organoids under various oxygen levels and found that treatment responses were altered under the different oxygen levels.

Taken together, their findings highlight the importance of modulating experimental conditions to include relevant oxygen levels in drug response studies in PDAC, enabling a better understanding of treatment failure as well as opportunities to use Hypoxia-inducible factor (HIF) prolyl hydroxylase (PHD) inhibitors or HIF inhibitors for the treatment of PDAC.


Go to the publication Sign up for our Newsletter

Cookies

‘May we use cookies?
Hi there! Thanks for visiting our website. We use cookies to keep track of our website statistics to optimize the user experience. We also use cookies for marketing purposes. You can set your preferences by selecting the options below. Terms of Use & Privacy Policy

Accept all
Accept selected
Decline all