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  • Expand your drug discovery capacity, shoulder to shoulder with our scientific team. Proprietary human disease biology in the OrganoPlate® platform. Together we make the therapeutics of tomorrow.
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  • Get robust compound data in human tissue models through our services. With proven pheno­typic assays in the OrganoPlate® platform, we support your drug discovery and development needs.
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  • Layered tissues with perfused tubules in the absence of artificial membranes form the heart of our permeability and transport science. Study cell interactions, permeability, absorption, transport, and transcytosis without physical barriers.

  • Co-culture layered & structured tissues without artificial membranes with perfect imaging, to study barrier-free cellular interactions, cell-cell signaling, and migration.

  • Evaluate the effect of chemotactic triggers or cells on the migration of cells through an extracellular matrix.

  • Membrane-free microvascular formation and growth through an extracellular matrix (ECM).

  • The missing link in tissue culture: add perfusable human vasculature to your tissue models, and recreate sophisticated microenvironments with OrganoPlate® Graft.

  • OrganoPlate® enables you to study relevant 3D tissue biology by incorporating perfused tubules, co-culture, and full control over the tissue microenvironment. Find the overview of applications here.

  • Visit our Knowledge Center to get up to speed with 3D tissue culture and to learn how OrganoPlate® supports your research needs.

    Read our publications, application notes, watch our webinars, or check out the supporting protocols and brochures. All compiled for you, by our scientists.

  • Get inspired by peer-reviewed publications of our scientists, partners, and customers around the globe.

  • Get inspired by research done by our scientists, partners, and customers around the globe.

  • Giving you some food for thought. Read our blogs to learn more about 3D tissue culture, research backgrounds, developments, and its future outlook.
  • Kickstart your experiments with the most sophisticated 3D tissue culture platform. Find out which training fits your 3D tissue modeling needs best.
  • Any support questions about purchasing, products, or 3D tissue culture and analysis? Get in touch with our experts.
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EN

A versatile multiplexed assay to quantify intracellular ROS and cell viability in 3D on-a-chip models

Leiden, September 30, 2022 – Scientists from MIMETAS develop a multiplexed assay to quantify reactive oxidative species (ROS) and cell viability in organ-on-a-chip models. This article has just been published in the Journal of Redox Biology.

Reactive oxygen species (ROS) are natural by-products of cellular oxidative metabolism. At low levels, ROS plays a key role in the processes such as regulation of cell proliferation, cell death, differentiaion, vasular tone, as well as angiogenesis. However, at high concentrations, ROS are harmful molecules that oxidize macromolecules, causing structural damage to cells and eventually apoptosis. This process is called oxidative stress (OS) and is not completely understood. Intracellular accumulation of ROS contributes and is implicated in many detrimental conditions including endothelial dysfunction, atherosclerosis, aging, and cancer. To better understand the role of OS and ROS in physiological and pathophysiological processes, quantifying ROS is of utmost importance.

Unfortunately, the quantification of ROS in vivo is challenging and is limited to in vitro studies due to its highly reactive nature, short half-life, and localization in discrete sub-cellular compartments. Overcoming these challenges warrants physiologically relevant in vitro models, which are currently lacking. The use of more advanced in vitro cell culture techniques such as 3D cell cultures or Organ-on-a-Chip, rather than simple 2D models would enable better recapitulation of the complexity and physiology typical of the in vivo situation.

In this publication, the authors describe the development, validation and application of a scalable multiplexed versatile live-cell and image-based assay to quantify ROS accumulation or depletion together with cell viability in 3D organ-on-a-chip in vitro models developed in the OrganoPlate®. Live cells were stained for ROS, dead cells, and DNA and confocal images were analyzed to quantify ROS probes and determine the number of nuclei and dead cells. Interestingly, the results showed that on-a-chip models are more prone to scavenge ROS rather than accumulate them, which is in line with previous studies where they compared OS profile in on-a-chip and 2D cell culture models. Moreover, the assay demonstrated high sensitivity, distinguishing between different phenotypes of endothelial cells based on the level of OS to detect higher level in tumor than normal cells. Taken together, this assay is a valuable tool for envisioned to enable the unravelling of mechanisms behind OS and ROS accumulation.

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