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  • Expand your drug discovery capacity, shoulder to shoulder with our scientific team. Proprietary human disease biology in the OrganoPlate® platform. Together we make the therapeutics of tomorrow.
  • We create novel human tissue and disease models in the OrganoPlate® platform. Our experts are looking forward to developing assays according to your specifications.
  • Get robust compound data in human tissue models through our services. With proven pheno­typic assays in the OrganoPlate® platform, we support your drug discovery and development needs.
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  • Layered tissues with perfused tubules in the absence of artificial membranes form the heart of our permeability and transport science. Study cell interactions, permeability, absorption, transport, and transcytosis without physical barriers.

  • Co-culture layered & structured tissues without artificial membranes with perfect imaging, to study barrier-free cellular interactions, cell-cell signaling, and migration.

  • Evaluate the effect of chemotactic triggers or cells on the migration of cells through an extracellular matrix.

  • Membrane-free microvascular formation and growth through an extracellular matrix (ECM).

  • The missing link in tissue culture: add perfusable human vasculature to your tissue models, and recreate sophisticated microenvironments with OrganoPlate® Graft.

  • OrganoPlate® enables you to study relevant 3D tissue biology by incorporating perfused tubules, co-culture, and full control over the tissue microenvironment. Find the overview of applications here.

  • Visit our Knowledge Center to get up to speed with 3D tissue culture and to learn how OrganoPlate® supports your research needs.

    Read our publications, application notes, watch our webinars, or check out the supporting protocols and brochures. All compiled for you, by our scientists.

  • Get inspired by peer-reviewed publications of our scientists, partners, and customers around the globe.

  • Get inspired by research done by our scientists, partners, and customers around the globe.

  • Giving you some food for thought. Read our blogs to learn more about 3D tissue culture, research backgrounds, developments, and its future outlook.
  • Kickstart your experiments with the most sophisticated 3D tissue culture platform. Find out which training fits your 3D tissue modeling needs best.
  • Any support questions about purchasing, products, or 3D tissue culture and analysis? Get in touch with our experts.
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EN

High-throughput 3D microvessel-on-a-chip model to study defective angiogenesis in systemic sclerosis

Leiden, October 10, 2022 Scientists from MIMETAS and Galapagos used a high-throughput 3D microvessel-on-a-chip on the OrganoPlate platform to study angiogenesis in the context of systemic sclerosis. This article has just been published in Scientific Reports.

Endothelial cells play a central role in the control of vascular function and is involved in all aspects of vascular homeostasis as well as in physiological or pathological processes like thrombosis, inflammation, or vascular wall remodeling. In systemic scleroris (SSc), a chronic autoimmune connective tissue disease, endothelial damage is an early and central event and leads to vascular impairment. Consequently, angiogenesis is dysregulated in SSc and therefore cannot facilitate efficient vascular recovery. The exact cause and mechanisms behind this endothelial damage remains unclear. Therefore, physiologically relevant models are urgently needed to study angiogenesis in SSc and to better understand the etiology behind the vascular impairment seen.

Most experimental systems used to study angiogenesis in SSc rely on animal models that do not always translate to human condition, are ethically problematic and are costly. Importantly, most cell culture models use fetal bovine serum which is not physiologically relevant. Traditional 2D in vitro models have limited throughput and demonstrate short-term stability of the capillary-like structures; these models also do not recapitulate the typical hallmarks of endothelial cells during angiogenesis in vivo such as lumen formation and differentiation into tip and stalk cells.

In this publication, a 3D angiogenesis assay/microvessel-on-a-chip to study systemic defective angiogenesis in SSc is described. This in vitro assay allows the study and monitoring of all stages of angiogenesis, from sprouts formation to sprouts stabilization and degradation in the presence of different triggers and compounds. To further increase the relevance of the model, HMVEC angiogenic sprouting assay was optimized to be compatible with human derived serum, which enabled a unique study on the effect of SSc patient derived serum on the stability of sprouts.

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