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  • Expand your drug discovery capacity, shoulder to shoulder with our scientific team. Proprietary human disease biology in the OrganoPlate® platform. Together we make the therapeutics of tomorrow.
  • We create novel human tissue and disease models in the OrganoPlate® platform. Our experts are looking forward to developing assays according to your specifications.
  • Get robust compound data in human tissue models through our OrganoServices. With proven pheno­typic assays in the OrganoPlate® platform, we support your drug discovery and development needs.
  • We offer several services to support your drug discovery and development needs. Find the overview here.
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  • Layered tissues with perfused tubules in the absence of artificial membranes form the heart of our permeability and transport science. Study cell interactions, permeability, absorption, transport, and transcytosis without physical barriers.

  • Co-culture layered & structured tissues without artificial membranes with perfect imaging, to study barrier-free cellular interactions, cell-cell signaling, and migration.

  • Evaluate the effect of chemotactic triggers or cells on the migration of cells through an extracellular matrix.

  • Membrane-free microvascular formation and growth through an extracellular matrix (ECM).

  • The missing link in tissue culture: add perfusable human vasculature to your tissue models, and recreate sophisticated microenvironments with OrganoPlate® Graft.

  • OrganoPlate® enables you to study relevant 3D tissue biology by incorporating perfused tubules, co-culture, and full control over the tissue microenvironment. Find the overview of applications here.

  • Visit our Knowledge Center to get up to speed with 3D tissue culture and to learn how OrganoPlate® supports your research needs.

    Read our publications, application notes, watch our webinars, or check out the supporting protocols and brochures. All compiled for you, by our scientists.

  • Get inspired by peer-reviewed publications of our scientists, partners, and customers around the globe.

  • Get inspired by research done by our scientists, partners, and customers around the globe.

  • Giving you some food for thought. Read our blogs to learn more about 3D tissue culture, research backgrounds, developments, and its future outlook.
  • Kickstart your experiments with the most sophisticated 3D tissue culture platform. Find out which training fits your 3D tissue modeling needs best.
  • Any support questions about purchasing, products, or 3D tissue culture and analysis? Get in touch with our experts.
  • Thinking of using OrganoPlate for your research? Request a quote for the product(s) of your interest.

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EN

A novel in vitro membrane permeability methodology using three-dimensional Caco-2 tubules in a microphysiological system

Leiden, November 23, 2021 – Scientists from MIMETAS and Astellas introduce a new method to study in vitro drug permeability based on 3D Caco-2 tubules cultured in the OrganoPlate® 3 lane-40. This article has just been published in the Journal of Pharmaceutical Sciences.

Drug absorption in the intestine is a critical step in orally-administered therapies. After oral dosing, the drug has to travel the gastrointestinal tract and be absorbed by the intestine into the bloodstream before it can be distributed to the site of action. By studying the mechanism of absorption, researchers can better predict the in vivo performance of novel drug compounds.

Current methods the pharmaceutical industry uses to study drug absorption by the intestine include the parallel artificial membrane permeability assay (PAMPA) and the Ussing Chamber model. The Ussing chamber model makes use of the complete structure of the human intestine. Even this model has high predictability, it suffers from technical and ethical issues as the intestinal tissues need to be sourced from human subjects. PAMPA on the other hand, only allows the researcher to determine passive diffusion of the drug compound across an artificial membrane. Considering the substantial drawbacks of the methods currently used, organ-on-a-chip technology can offer an impactful alternative for drug researchers to study intestinal absorption.

This publication set out to conduct a proof-of-concept study of a novel in vitro drug absorption method that employs a 3D-cultured intestine-on-a-chip model. By culturing Caco-2 cells in the OrganoPlate® 3-lane 40, distinct intestinal tubules were formed with both apical and basolateral access. The apical side of the intestinal tube was coated with a mucus layer to protect the Caco-2 cells from bile acid toxicity, which allows the researchers to determine the effect of bile acid on intestinal absorption without damaging the intestinal tissues. With the use of propranolol and solifenacin as exemplar compounds, the scientists show that the model can serve as an alternative testing system for measuring drug absorption in an environment closely resembling that of the human gastrointestinal tract.

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