• Get inspired by peer-reviewed publications of our scientists, partners, and customers around the globe.

  • Giving you some food for thought. Read our blogs to learn more about 3D tissue culture, research backgrounds, developments, and its future outlook.
  • Get inspired by research done by our scientists, partners, and customers around the globe.

  • Learn about our mission, vision, the history of the company, and find out what we mean with MIMETAS-do.
open menu icon close menu icon

Snake Venom Gland Organoids

Unlocking possibilities for more effective production of bioactive compounds from snake venom

Leiden, January 23, 2020– The Hubrecht institute and MIMETAS investigated if venom molecules produced by snake venom gland organoids also function as actual venom using the OrganoPlate® platform.

Although snake bites are not very common in developed countries, snakebite envenoming is still estimated to be responsible for more than 100,000 deaths worldwide each year. The composition of snake venom differs greatly between snake species, requiring treatment with different antidotes for bites from different snakes. Treatment of snake envenoming is further complicated by logistical difficulties. Antidote production is expensive, labor-intensive, and not animal friendly as it requires keeping and milking of snakes, immunization of other animals, and collection and purification of the produced antibodies.

Researchers at Hubrecht institute have developed a method that may circumvent many of the issues encountered with the current snake venom treatments. Researchers in the lab of Hans Clevers isolated stem cells from the venom gland of the venomous cape coral snake. From these venom gland stem cells, they grew organoids: three-dimensional cell structures that show similarities to the actual venom gland of the cape coral snake. Using various techniques, the work published in Cell shows that the snake venom gland organoids produce many of the same molecules found in the venom of adult cape coral snakes.

The Hubrecht institute teamed up with MIMETAS to assess whether the venom molecules produced by the snake venom gland organoids also function as actual venom. MIMETAS used its OrganoPlate® platform to culture muscle fibers that express the nicotinic acetylcholine receptor. The team at MIMETAS showed that muscle fibers that were exposed to the venom molecules from the organoids were no longer able to respond to a molecule resembling acetylcholine. This indicates that the venom molecules from the organoids are likely binding to the acetylcholine receptor the same way that toxins from adult snakes do. The study published in Cell is the first to apply organoid technology to non-vertebrate species and opens up possibilities for easier, cheaper, and more animal friendly production of snake venom antidotes and other bioactive compounds present in snake venom.

Video 1: C2C12 muscle cells that weren't exposed to supernatant from the snake venom
organoids show calcium wave propagation in response to acetyl choline receptor agonist
carbachol (the movement of the green signal).

Video 2: Cells that were exposed to snake venom organoid supernatant don't respond
to carbachol (no wave propagation observed)

About Cell-cell interactions Our Services OrganoPlate® 2-lane


‘May we use cookies?
Hi there! Thanks for visiting our website. We use cookies to keep track of our website statistics to optimize the user experience. We also use cookies for marketing purposes. You can set your preferences by selecting the options below. Terms of Use & Privacy Policy

Accept all
Accept selected
Decline all