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  • About Mimetas
  • Supporting up to 96 tissue models on a single plate. Its unique PhaseGuide™ technology enables cells to interact and migrate freely between the two channels of each chip.

  • Supporting up to 40 tissue models on a single plate. Its unique PhaseGuide™ technology enables cells to interact and migrate freely between the three channels of each chip.
  • Supporting up to 64 tissue models on a single plate. Specially designed for automated workflows. Its unique PhaseGuide™ technology enables cells to interact and migrate freely between the three channels of each chip.
  • The first in vitro tissue culture platform that allows co-culture of spheroids, organoids, and tumors with a perfused microvascular bed and vascularization of 3D tissues.

  • Offering 38 ready-to-use Caco-2 tubules for drug exposure, transport, and permeability studies.
  • Driving precisely-controlled perfusion flow in the OrganoPlate® platform
  • Enabling fast, automated, and impedance-based TEER measurements in OrganoPlate®. Assay 40 tissue culture chips with a few clicks, in less than one minute.
  • Kickstart your experiments with the most sophisticated 3D tissue culture platform. Find out which training fits your 3D tissue modeling needs best.
  • OrganoPlate® is the solution for all in vitro tissue culture applications. Explore the entire OrganoPlate® family and its dedicated instruments here.

  • Get robust compound data in human tissue models. With proven pheno­typic assays in the OrganoPlate® platform, we support your drug discovery and development needs.
  • We create novel human tissue and disease models in the OrganoPlate® platform. Our experts are looking forward to developing assays according to your specifications.
  • Expand your drug discovery capacity, shoulder to shoulder with our scientific team. Proprietary human disease biology in the OrganoPlate® platform. Together we make the therapeutics of tomorrow.
  • We offer several services to support your drug discovery and development needs. Find the overview here.
  • Layered tissues with perfused tubules in the absence of artificial membranes form the heart of our permeability and transport science. Study cell interactions, permeability, absorption, transport, and transcytosis without physical barriers.

  • Co-culture layered & structured tissues without artificial membranes with perfect imaging, to study barrier-free cellular interactions, cell-cell signaling, and migration.

  • Evaluate the effect of chemotactic triggers or cells on the migration of cells through an extracellular matrix.

  • Membrane-free microvascular formation and growth through an extracellular matrix (ECM).

  • The missing link in tissue culture: add perfusable human vasculature to your tissue models, and recreate sophisticated microenvironments with OrganoPlate® Graft.

  • OrganoPlate® enables you to study relevant 3D tissue biology by incorporating perfused tubules, co-culture, and full control over the tissue microenvironment. Find the overview of applications here.

  • Visit our Knowledge Center to get up to speed with 3D tissue culture and to learn how OrganoPlate® supports your research needs.

    Read our publications, application notes, watch our webinars, or check out the supporting protocols and brochures. All compiled for you, by our scientists.

  • Get inspired by peer-reviewed publications of our scientists, partners, and customers around the globe.

  • Get inspired by research done by our scientists, partners, and customers around the globe.

  • Giving you some food for thought. Read our blogs to learn more about 3D tissue culture, research backgrounds, developments, and its future outlook.
  • Kickstart your experiments with the most sophisticated 3D tissue culture platform. Find out which training fits your 3D tissue modeling needs best.
  • Any support questions about purchasing, products, or 3D tissue culture and analysis? Get in touch with our experts.
  • Thinking of using OrganoPlate for your research? Request a quote for the product(s) of your interest.

  • Frequently asked questions answered by our experts. Find here the answer you're looking for.
  • Learn about our mission, vision, the history of the company, and find out what we mean with MIMETAS-do.
ENGLISH

Snake Venom Gland Organoids

Unlocking possibilities for more effective production of bioactive compounds from snake venom


Leiden, January 23, 2020– The Hubrecht institute and MIMETAS investigated if venom molecules produced by snake venom gland organoids also function as actual venom using the OrganoPlate® platform.

Although snake bites are not very common in developed countries, snakebite envenoming is still estimated to be responsible for more than 100,000 deaths worldwide each year. The composition of snake venom differs greatly between snake species, requiring treatment with different antidotes for bites from different snakes. Treatment of snake envenoming is further complicated by logistical difficulties. Antidote production is expensive, labor-intensive, and not animal friendly as it requires keeping and milking of snakes, immunization of other animals, and collection and purification of the produced antibodies.

Researchers at Hubrecht institute have developed a method that may circumvent many of the issues encountered with the current snake venom treatments. Researchers in the lab of Hans Clevers isolated stem cells from the venom gland of the venomous cape coral snake. From these venom gland stem cells, they grew organoids: three-dimensional cell structures that show similarities to the actual venom gland of the cape coral snake. Using various techniques, the work published in Cell shows that the snake venom gland organoids produce many of the same molecules found in the venom of adult cape coral snakes.

The Hubrecht institute teamed up with MIMETAS to assess whether the venom molecules produced by the snake venom gland organoids also function as actual venom. MIMETAS used its OrganoPlate® platform to culture muscle fibers that express the nicotinic acetylcholine receptor. The team at MIMETAS showed that muscle fibers that were exposed to the venom molecules from the organoids were no longer able to respond to a molecule resembling acetylcholine. This indicates that the venom molecules from the organoids are likely binding to the acetylcholine receptor the same way that toxins from adult snakes do. The study published in Cell is the first to apply organoid technology to non-vertebrate species and opens up possibilities for easier, cheaper, and more animal friendly production of snake venom antidotes and other bioactive compounds present in snake venom.


Video 1: C2C12 muscle cells that weren't exposed to supernatant from the snake venom
organoids show calcium wave propagation in response to acetyl choline receptor agonist
carbachol (the movement of the green signal).


Video 2: Cells that were exposed to snake venom organoid supernatant don't respond
to carbachol (no wave propagation observed)


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