Cell migration is an essential process for many biological phenomena such as early embryonic development and immune cell response. During inflammation, the recruitment and migration of T cells through the vascular system towards the site of inflammation is a crucial component.
In this app note, we demonstrate the development of an in vitro assay utilizing the OrganoPlate® platform to study T cell dynamics in combination with a blood vessel structure under perfused conditions, as well as the high-content imaging used to observe the migration of cells at various time points.
Benefits of the model:
- Create complex cell migration assays that are easily scalable and compatible for high-content imaging
- Perform quantitative assessment of immune cell dynamics, generating high throughput readouts such as cell numbers and cell migration distance