One of the crucial steps in the human immune response is the migration of T cells from an endothelial vessel into the surrounding tissue. If this process is aberrant, it could lead to an improper immune response due to a lack of T cells, or chronic inflammation in excess of T cells, which makes it an important aspect of study.
Whereas in vivo models suffer from a lack of translational value and increasing ethical debate, simplified in vitro models lack vasculature, extracellular matrix (ECM), and neighboring cells, leading to suboptimal research models. Organ-on-a-chip models can provide valuable solutions to these issues.
In this application note we show our newly developed T cell perfused endothelium on a chip model to study transendothelial migration of T cells towards chemotactic triggers and tumor cells.
Benefits of the model:
- A robust and endothelium-on-a-chip model to study T cell migration in the absence of artificial membranes
- Induction of T cell migration based on a chemotactic trigger and tumor cells
- Suitable for high throughput screening of migration inducing or inhibiting compounds