Understanding intestinal permeability is a critical step in evaluating drug absorption, bioavailability, and safety. This eBook provides a comprehensive guide to building physiologically relevant 3D in vitro intestinal models using Organ-on-a-Chip technology.Drawing from real-world examples and validated protocols, it outlines:The biological relevance of 3D intestinal models compared to conventional 2D systemsHow to mimic in vivo-like architecture and function using perfused microfluidic platformsMethods to assess drug transport, barrier integrity, and compound-induced toxicityKey considerations for model setup, culture conditions, and experimental readoutsThe content is especially useful for scientists and researchers working in preclinical drug development, ADME-Tox, and translational biology. Whether you're optimizing compound screening workflows or replacing animal models, this resource supports more predictive and human-relevant data generation.